PXD019396
PXD019396 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | SCFCyclin F targets the metabolic protein Sirtuin 5 for ubiquitination |
Description | Cell cycle transitions are regulated by a number of coordinating signaling events. The ubiquitin-proteasome system is an important part of this cell cycle signaling. Cyclin F, a substrate adapter for the SCF (SKP1/CUL1/F-box) E3 ubiquitin ligase, is an important regulator of cell cycle progression. However, identifying SCFCyclin F substrates important for the G1-S transition and other cell cycle events has remained technically challenging. Since Cyclin F overexpression rescues a yeast mutant in the cdc4 gene, we considered the possibility that other genes that genetically modify cdc4 mutant lethality could also encode Cyclin F substrates. We identified the mitochondrial and cytosolic deacylating enzyme Sirtuin 5 (SIRT5) as a novel Cyclin F substrate. SIRT5 has been implicated in a number of metabolic processes, but its connection to the cell cycle is not known. Here, we demonstrate the ability of Cyclin F to interact with, ubiquitinate and control the abundance and stability of SIRT5. Since previously identified Cyclin F targets are important for cell cycle progression, we hypothesized that SIRT5 would influence cell cycle progression. We show SIRT5 CRISPR knockout results in a diminished G1 population, and subsequent increase in both S and G2/M. Proteomic analyses reveal CDK signaling changes congruent with the cell cycle changes in SIRT5 knockout cells. Together these data demonstrate SIRT5 is regulated by Cyclin F and suggest a connection between SIRT5, cell cycle regulation, and metabolism. |
HostingRepository | jPOST |
AnnounceDate | 2020-11-17 |
AnnouncementXML | Submission_2024-05-09_11:33:14.614.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Grimsrud |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-26 13:06:26 | ID requested | |
1 | 2020-11-17 06:18:42 | announced | |
2 | 2021-02-04 16:56:41 | announced | 2021-02-04: Updated FTP location. |
3 | 2022-09-18 03:51:34 | announced | 2022-09-18: Updated FTP location. |
⏵ 4 | 2024-05-09 11:33:16 | announced | 2024-05-10: Updated PubMed. |
Publication List
Mills CA, Wang X, Bhatt DP, Grimsrud PA, Matson JP, Lahiri D, Burke DJ, Cook JG, Hirschey MD, Emanuele MJ, Ubiquitin Ligase and Is Involved in Cell Cycle Control. Mol Cell Biol, 41(2):(2021) [pubmed] |
Keyword List
submitter keyword: Cyclin F (CCNF), Sirtuin 5 (SIRT5), cell cycle, ubiquitin, metabolism |
Contact List
Matthew D. Hirschey | |
---|---|
lab head | |
Paul Grimsrud | |
contact affiliation | Duke University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000855/ |