PXD019395

PXD019395 is an original dataset announced via ProteomeXchange.

Title	Atherosclerotic environment effect over CAC pre-angiogenic role
Description	In response to vascular injury, early EPC or Circulating angiogenic cells (CAC) are thought to be recruited to the damaged areas, participating mainly in a paracrine fashion, by releasing pro-angiogenic factors, promoting the mobilization of other cell populations such as the late EPC, or endothelial colony-forming cells (ECFC). ECFC will help by replacing the damaged endothelium and promoting neovascularization. Unfortunately, however, despite the regenerative role, the number and function of EPC are severely affected under pathological conditions, making compulsory to better understand how these cells react under those environments in order to implement their use in regenerative cell therapies. Herein we evaluated, for the first time to our knowledge, the effect of atherosclerotic factors over the paracrine role of CAC, by direct incubation ex vivo of healthy CAC with the secretome of atherosclerotic arteries. The application of a label free proteomics approach allowed the identification of 194 altered proteins in the secretome of pre-conditioned CAC, many of them related, among others, with inhibition of angiogenesis and cell migration. Functional assays corroborated that, after atherosclerotic pre-conditioning, the pro-angiogenic effect of CAC over ECFC was impaired, affecting ECFC migration as well as their ability to form tubules on a basement membrane matrix assay. Up-regulation of several anti-angiogenic proteins in the CAC secretome could help to explain such angiogenic switch which, in turns, could reflect a protective and beneficial effect against the atherosclerotic process.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:49.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carmen Duran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 07:43:20	ID requested
1	2021-09-08 23:40:40	announced
⏵ 2	2024-10-22 05:27:50	announced	2024-10-22: Updated project metadata.

Eslava-Alcon S, Extremera-Garc, í, a MJ, Sanchez-Gomar I, Beltr, á, n-Camacho L, Rosal-Vela A, Mu, ñ, oz J, Ibarz N, Alonso-Pi, ñ, ero JA, Rojas-Torres M, Jim, é, nez-Palomares M, Gonz, á, lez-Rovira A, Conejero R, Doiz E, Rodriguez-Pi, ñ, ero M, Moreno-Luna R, Dur, á, n-Ruiz MC, Atherosclerotic Pre-Conditioning Affects the Paracrine Role of Circulating Angiogenic Cells Ex-Vivo. Int J Mol Sci, 21(15):(2020) [pubmed]

10.3390/ijms21155256;

submitter keyword: proteomics, angiogenesis,Circulating angiogenic cells, atherosclerotic factors, paracrine role, endothelial colony forming cells, secretome

MªCarmen Duran-Ruiz
contact affiliation	University of Cadiz
contact email	maricarmen.duran@gm.uca.es
lab head
Carmen Duran
contact affiliation	University of Cadiz
contact email	maricarmen.duran@gm.uca.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019395

PRIDE project URI

• PRIDE
  1. PXD019395
     1. Label: PRIDE project
     2. Name: Atherosclerotic environment effect over CAC pre-angiogenic role