PXD019393

PXD019393 is an original dataset announced via ProteomeXchange.

Title	Complement deposition on Chlamydia trachomatis
Description	Introduction Chlamydia trachomatis (C. trachomatis) is a Gram-negative bacterium and a common human pathogen. The World Health Organization (WHO) estimates that over 130 million people are infected with C. trachomatis globally each year and with increasing incidence. C. trachomatis causes long-lasting and recurrent infections that over time induce severe tissue damage in the female genital tract that can lead to ectopic pregnancy and infertility. Thus, the human immune system fails to control and eradicate C. trachomatis during primary infection and fails to develop protective immunity against secondary infections. In vivo infection models, using complement knock out mice, suggest that the complement system is critically involved in both anti-chlamydial immunity and infection-induced pathology. To increase our understanding of complement-mediated immunity against C. trachomatis we analyzed global complement deposition on serum-incubated C. trachomatis by mass spectrometry. Methods Purified C. trachomatis was incubated in seronegative normal human serum (NHS) or heat-inactivated normal human serum (HI-NHS) for 30 min, thoroughly washed, and processed for mass spectrometry. All samples were lysed, reduced and alkylated and digested with trypsin. Some samples were chemically modified to acetylate free amino groups (N-terminal and lysine amino groups) before trypsin digestion. Peptides were analyzed on a UltimateTM 3500 RSLCnano coupled to a Q Exactive HF-X mass spectrometer. Raw data files were searched against the Uniprot human reference proteome using MaxQuant. Results We demonstrate that C. trachomatis elicits potent complement activation demonstrated by deposition of both early and late complement factors together with several complement regulators. We further demonstrate proteolytically processing of complement C3b to “inactive” C3 cleavage fragments. Conclusion We demonstrate the deposition of several novel complement-associated proteins and -cleavage fragments on the surface of C. trachomatis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:29.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tue Bjerg Bennike
SpeciesList	scientific name: Chlamydia trachomatis L2/434/Bu(f); NCBI TaxID: 1262673; scientific name: Chlamydia trachomatis; NCBI TaxID: 813; scientific name: Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B); NCBI TaxID: 471472; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlamydia trachomatis L2/434/Bu(i); NCBI TaxID: 1263406;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 05:40:25	ID requested
1	2020-11-23 07:35:43	announced
⏵ 2	2024-10-22 05:15:35	announced	2024-10-22: Updated project metadata.

10.1007/s00430-020-00695-x;

Lausen M, Thomsen ME, Christiansen G, Karred N, Stensballe A, Bennike TB, Birkelund S, Analysis of complement deposition and processing on Chlamydia trachomatis. Med Microbiol Immunol, 210(1):13-32(2021) [pubmed]

submitter keyword: C3, complement, human, plasma,Chlamydia trachomatis, LC-MS/MS

Svend Birkelund
contact affiliation	Head of Medical Microbiology and Immunology, Department of Health Science and Technology, Medical Faculty, Aalborg Universtity, Denmark
contact email	sbirkelund@hst.aau.dk
lab head
Tue Bjerg Bennike
contact affiliation	Aalborg University
contact email	tbe@hst.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019393
     1. Label: PRIDE project
     2. Name: Complement deposition on Chlamydia trachomatis