PXD019392

PXD019392 is an original dataset announced via ProteomeXchange.

Title	Changes in composition and levels of hemolymph proteins provide insights to their functions in pharate, early and late pupae and adults of Manduca sexta
Description	The tobacco hornworm, Manduca sexta, is a lepidopteran model species widely used to study insect biochemical processes. While some of its larval hemolymph proteins are well understood, a detailed proteomic analysis was unavailable until 2016, revealing features such as correlation with transcriptome data, formation of immune complexes, and constitution of an immune signaling system. Yet, it is unclear how these may change in other developmental stages. In this paper, we report the proteomes of cell-free hemolymph from prepupae, pupae on days 4 and 13, and young adults. Of the 1,824 proteins identified, 907 have a signal peptide and 215 are related to immunity. Drastic changes in abundance of the storage proteins, for instance, reflect physiological disparities among prepupae, pupae, and adults. Considerably more proteins lacking signal peptide are present in the late pupae, suggesting that plasma acts as a temporary reservoir for intracellular components released from remodeling tissues during metamorphosis. In summary, the proteins and their levels revealed in this study are expected to stimulate focused explorations of humoral immunity in wandering larvae, pupae, and adults of M. sexta and shed light upon functional and comparative genomic research in other holometabolous insects.
HostingRepository	PRIDE
AnnounceDate	2020-10-30
AnnouncementXML	Submission_2020-10-30_01:22:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaolong Cao
SpeciesList	scientific name: Manduca sexta; NCBI TaxID: 7130;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 03:52:58	ID requested
⏵ 1	2020-10-30 01:22:03	announced

Cao X, Wang Y, Rogers J, Hartson S, Kanost MR, Jiang H, Changes in composition and levels of hemolymph proteins during metamorphosis of Manduca sexta. Insect Biochem Mol Biol, 127():103489(2020) [pubmed]

submitter keyword: manduca sexta, hemolymph, proteome, pupa, immunity

Haobo Jiang
contact affiliation	Regents Professor Department of Entomology & Plant Pathology Oklahoma State University, Stillwater, OK 74078 Telephone: 405-744-9400
contact email	Haobo.Jiang@okstate.edu
lab head
Xiaolong Cao
contact affiliation	Oklahoma State University
contact email	xiaolong.cao@okstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019392
     1. Label: PRIDE project
     2. Name: Changes in composition and levels of hemolymph proteins provide insights to their functions in pharate, early and late pupae and adults of Manduca sexta