PXD019391

PXD019391 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Deciphering the function of Angiomotin in trophoblast cells
Description	Trophectoderm-specific expression of Angiomotin (AMOT) in pre-implantation embryos followed by its unique expression in the post-implantation ectoplacental cone that harbors the trophoblast stem cell niche prompted our investigation on the function of AMOT in trophoblast cells. Using the in vitro trophoblast stem cell culture model, we established differentiation dependent up-regulation of AMOT expression in trophoblast cells. To understand the function of AMOT in trophoblast cells mass spectrometry-based proteomic analysis was employed to identify the AMOT interactome within the trophoblast proteome. This approach utilized immunoprecipitation of endogenous AMOT followed by fractionation on SDS-PAGE and subsequently subjecting the tryptic digested excised gel bands to mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:04.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TUSHAR KANTI MAITI
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 03:52:44	ID requested
1	2021-11-03 05:20:32	announced
⏵ 2	2024-10-22 05:30:05	announced	2024-10-22: Updated project metadata.

Publication List

10.1002/stem.3305;
Basak T, Dey AK, Banerjee R, Paul S, Maiti TK, Ain R, Sequestration of eIF4A by angiomotin: A novel mechanism to restrict global protein synthesis in trophoblast cells. Stem Cells, 39(2):210-226(2021) [pubmed]

10.1002/stem.3305;

Basak T, Dey AK, Banerjee R, Paul S, Maiti TK, Ain R, Sequestration of eIF4A by angiomotin: A novel mechanism to restrict global protein synthesis in trophoblast cells. Stem Cells, 39(2):210-226(2021) [pubmed]

Keyword List

submitter keyword: in-gel digestion,Angiomotin, exponentially modified protein abundance index (emPAI), normalized spectral abundance factor (NSAF)

submitter keyword: in-gel digestion,Angiomotin, exponentially modified protein abundance index (emPAI), normalized spectral abundance factor (NSAF)

Contact List

Dr Tushar Kanti Maiti
contact affiliation	Associate Professor Regional Centre for Biotechnology
contact email	tkmaiti@rcb.res.in
lab head
TUSHAR KANTI MAITI
contact affiliation	Regional Centre for Biotechnology
contact email	tkmaiti@rcb.res.in
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD019391
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD019391

PRIDE project URI

Repository Record List

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