PXD019386

PXD019386 is an original dataset announced via ProteomeXchange.

Title	The OPTN interactome using BioID
Description	We use a proximity labelling-based proteomics strategy (BioID) to map the interactome of OPTN. OPTN was tagged N and C-terminally with BirA* in RPE1 cells and then, after labelling with biotin, streptavidin pull downs were performed to identify OPTN-proximal proteins.
HostingRepository	PRIDE
AnnounceDate	2020-06-16
AnnouncementXML	Submission_2020-06-16_00:56:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas O'Loughlin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-26 01:11:43	ID requested
⏵ 1	2020-06-16 00:56:53	announced

O'Loughlin T, Kruppa AJ, Ribeiro ALR, Edgar JR, Ghannam A, Smith AM, Buss F, OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion. J Cell Sci, 133(12):(2020) [pubmed]

submitter keyword: BioID, OPTN

Folma Buss
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, UK
contact email	fb207@cam.ac.uk
lab head
Thomas O'Loughlin
contact affiliation	University of Cambridge
contact email	taoloughlin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019386

PRIDE project URI

• PRIDE
  1. PXD019386
     1. Label: PRIDE project
     2. Name: The OPTN interactome using BioID