PXD019381

PXD019381 is an original dataset announced via ProteomeXchange.

Title	An anatomic proteomic atlas of human glioblastoma
Description	Glioblastoma (GBM) is an aggressive form of brain cancer with well-established patterns of intra-tumoral heterogeneity implicated in treatment resistance, recurrence and progression. While the regional and single cell-level genetic variation of GBM have been recently resolved, downstream phenotype-level proteomic programs have yet to be assigned to specific niches. Here, we leverage laser capture microdissection and mass spectrometry-based proteomics to assign 4794 proteins to GBM’s hallmark histomorphologic niches across 20 patients. Importantly, this analysis defined 1360 regionally enriched proteins, including 502 which are proteogenomically concordant. We validate a subset of identified niche-specific markers using orthogonal immunohistochemical approaches and make the associated atlas publicly available as an online resource (https://www.brainproteinatlas.org/dash/apps/GPA). Spatial resolution of the molecular landscape of GBM, operational at the protein level, aims to further facilitate and refine our biological understanding and treatment approaches for this aggressive disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:57.212.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ugljesa Djuric
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-25 23:50:17	ID requested
1	2021-11-02 06:56:12	announced
2	2023-04-11 06:33:03	announced	2023-04-11: Updated project metadata.
⏵ 3	2024-10-22 05:30:05	announced	2024-10-22: Updated project metadata.

10.1038/s41467-021-27667-w;

Lam KHB, Leon AJ, Hui W, Lee SC, Batruch I, Faust K, Klekner A, Hut, ó, czki G, Koritzinsky M, Richer M, Djuric U, Diamandis P, Topographic mapping of the glioblastoma proteome reveals a triple-axis model of intra-tumoral heterogeneity. Nat Commun, 13(1):116(2022) [pubmed]

submitter keyword: Glioblastoma, laser capture microdissection,anatomical niches, LC-MS/MS

Phedias Diamandis
contact affiliation	University Health Network, Princess Margaret Hospital
contact email	Phedias.diamandis@uhn.ca
lab head
Ugljesa Djuric
contact affiliation	Mt. Sinai Hospital
contact email	ugljesa.djuric@mail.utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD019381

PRIDE project URI

• PRIDE
  1. PXD019381
     1. Label: PRIDE project
     2. Name: An anatomic proteomic atlas of human glioblastoma