PXD019374

PXD019374 is an original dataset announced via ProteomeXchange.

Title	Ablation of the miRNA cluster 24 has profound effects on extracellular matrix protein abundance in cartilage
Description	MicroRNAs (miRNAs) regulate cartilage differentiation and contribute to the onset and progression of joint degeneration. These small RNA molecules may affect extracellular matrix organization (ECM) in cartilage, but for only a few miRNAs has this role been defined in vivo. Previously, we showed that cartilage-specific genetic ablation of the Mirc24 cluster in mice leads to impaired cartilage development due to increased RAF/MEK/ERK pathway activation. Here, we studied the expression of the cluster in cartilage by LacZ reporter gene assays and determined its role for extracellular matrix homeostasis by proteome and immunoblot analysis. The cluster is expressed in prehypertrophic/hypertrophic chondrocytes of the growth plate and we now show that the cluster is also highly expressed in articular cartilage. Cartilage-specific loss of the cluster leads to increased proteoglycan 4 and matrix matrix metallopeptidase 13 levels and decreased aggrecan and collagen X levels in epiphyseal cartilage. Interestingly, these changes are linked to a decrease in SRY-related HMG box-containing (SOX) transcription factors 6 and 9, which regulate ECM production in chondrocytes. Our data suggests that the Mirc24 cluster is important for ECM homoeostasis and the expression of transcriptional regulators of matrix production in cartilage.
HostingRepository	PRIDE
AnnounceDate	2020-06-18
AnnouncementXML	Submission_2020-06-17_22:56:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronika S. Georgieva
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-25 04:02:29	ID requested
⏵ 1	2020-06-17 22:56:25	announced

Georgieva VS, Etich J, Bluhm B, Zhu M, Frie C, Wilson R, Zaucke F, Bateman J, Brachvogel B, Ablation of the miRNA Cluster 24 Has Profound Effects on Extracellular Matrix Protein Abundance in Cartilage. Int J Mol Sci, 21(11):(2020) [pubmed]

submitter keyword: extracellular matrix

Mirc24

miR-322

miR-503

cartilage

PRG4

articular

SOX9

SOX6

MMP13

Bent Brachvogel
contact affiliation	Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, Cologne, Germany and Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany
contact email	bent.brachvogel@uni-koeln.de
lab head
Veronika S. Georgieva
contact affiliation	Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, Cologne, Germany and Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany
contact email	veronika.georgieva@uk-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019374

PRIDE project URI

• PRIDE
  1. PXD019374
     1. Label: PRIDE project
     2. Name: Ablation of the miRNA cluster 24 has profound effects on extracellular matrix protein abundance in cartilage