⮝ Full datasets listing

PXD019367

PXD019367 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family
DescriptionG protein-coupled receptors (GPCRs) are the largest receptor superfamily that can propagate various extracellular stimulus into cells by coupling with heterotrimeric G proteins. G proteins are divided into four families, Gs, Gi/o, Gq/11 and G12/13, which are responsible for the transduction of discrete downstream signaling pathways. Interestingly, one receptor can couple to more than one G protein subtype with different coupling efficiency or kinetics; coupling with the highest efficiency and/or kinetics is known as ‘primary coupling’ whereas the one with lower efficiency and/or slower kinetics is known as ‘secondary coupling’. Due to its significance in human physiology, there has been a great effort to elucidate the precise mechanism of GPCR-G protein coupling, however, the complex nature of GPCR and G protein interaction raises more unanswered questions. Here, we utilized hydrogen/deuterium exchange mass spectrometry (HDX-MS) to understand the molecular mechanism underlying primary and secondary Gi/o coupling using muscarinic acetylcholine receptor type 2 (M2R) and β2-adrenergic receptor (β2AR) as the primary and secondary Gi/o-coupling receptors, respectively. Results showed the engagement of the distal C-terminus of Gi/o with the receptor differentiates primary and secondary Gi/o couplings. In addition, the interaction between the intracellular loop 2 (ICL2) of the receptor and Gi/o in primary Gi/o coupling is not as critical as in primary Gs coupling.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:08:21.486.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHee Ryung Kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentXevo G2 Q-Tof
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-25 04:00:38ID requested
12020-07-01 23:26:48announced
22024-10-22 05:08:23announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41467-020-16975-2;
Kim HR, Xu J, Maeda S, Duc NM, Ahn D, Du Y, Chung KY, Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family. Nat Commun, 11(1):3160(2020) [pubmed]
Keyword List
submitter keyword: primary couplling, secondary coupling,GPCR, G protein, HDX-MS
Contact List
Ka Young Chung
contact affiliationSchool of Pharmacy, Sungkyunkwan University, South Korea
contact emailkychung2@skku.edu
lab head
Hee Ryung Kim
contact affiliationHospital for Sick Children Research Institute
contact emailheeryung.jan.kim@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019367
PRIDE project URI
Repository Record List
[ + ]