PXD019367 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family |
Description | G protein-coupled receptors (GPCRs) are the largest receptor superfamily that can propagate various extracellular stimulus into cells by coupling with heterotrimeric G proteins. G proteins are divided into four families, Gs, Gi/o, Gq/11 and G12/13, which are responsible for the transduction of discrete downstream signaling pathways. Interestingly, one receptor can couple to more than one G protein subtype with different coupling efficiency or kinetics; coupling with the highest efficiency and/or kinetics is known as ‘primary coupling’ whereas the one with lower efficiency and/or slower kinetics is known as ‘secondary coupling’. Due to its significance in human physiology, there has been a great effort to elucidate the precise mechanism of GPCR-G protein coupling, however, the complex nature of GPCR and G protein interaction raises more unanswered questions. Here, we utilized hydrogen/deuterium exchange mass spectrometry (HDX-MS) to understand the molecular mechanism underlying primary and secondary Gi/o coupling using muscarinic acetylcholine receptor type 2 (M2R) and β2-adrenergic receptor (β2AR) as the primary and secondary Gi/o-coupling receptors, respectively. Results showed the engagement of the distal C-terminus of Gi/o with the receptor differentiates primary and secondary Gi/o couplings. In addition, the interaction between the intracellular loop 2 (ICL2) of the receptor and Gi/o in primary Gi/o coupling is not as critical as in primary Gs coupling. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:08:21.486.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hee Ryung Kim |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Xevo G2 Q-Tof |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-25 04:00:38 | ID requested | |
1 | 2020-07-01 23:26:48 | announced | |
⏵ 2 | 2024-10-22 05:08:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-020-16975-2; |
Kim HR, Xu J, Maeda S, Duc NM, Ahn D, Du Y, Chung KY, Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family. Nat Commun, 11(1):3160(2020) [pubmed] |
Keyword List
submitter keyword: primary couplling, secondary coupling,GPCR, G protein, HDX-MS |
Contact List
Ka Young Chung |
contact affiliation | School of Pharmacy, Sungkyunkwan University, South Korea |
contact email | kychung2@skku.edu |
lab head | |
Hee Ryung Kim |
contact affiliation | Hospital for Sick Children Research Institute |
contact email | heeryung.jan.kim@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019367 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019367
- Label: PRIDE project
- Name: Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family