PXD019367

PXD019367 is an original dataset announced via ProteomeXchange.

Title	Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family
Description	G protein-coupled receptors (GPCRs) are the largest receptor superfamily that can propagate various extracellular stimulus into cells by coupling with heterotrimeric G proteins. G proteins are divided into four families, Gs, Gi/o, Gq/11 and G12/13, which are responsible for the transduction of discrete downstream signaling pathways. Interestingly, one receptor can couple to more than one G protein subtype with different coupling efficiency or kinetics; coupling with the highest efficiency and/or kinetics is known as ‘primary coupling’ whereas the one with lower efficiency and/or slower kinetics is known as ‘secondary coupling’. Due to its significance in human physiology, there has been a great effort to elucidate the precise mechanism of GPCR-G protein coupling, however, the complex nature of GPCR and G protein interaction raises more unanswered questions. Here, we utilized hydrogen/deuterium exchange mass spectrometry (HDX-MS) to understand the molecular mechanism underlying primary and secondary Gi/o coupling using muscarinic acetylcholine receptor type 2 (M2R) and β2-adrenergic receptor (β2AR) as the primary and secondary Gi/o-coupling receptors, respectively. Results showed the engagement of the distal C-terminus of Gi/o with the receptor differentiates primary and secondary Gi/o couplings. In addition, the interaction between the intracellular loop 2 (ICL2) of the receptor and Gi/o in primary Gi/o coupling is not as critical as in primary Gs coupling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:21.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hee Ryung Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Xevo G2 Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-25 04:00:38	ID requested
1	2020-07-01 23:26:48	announced
⏵ 2	2024-10-22 05:08:23	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-16975-2;

Kim HR, Xu J, Maeda S, Duc NM, Ahn D, Du Y, Chung KY, Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family. Nat Commun, 11(1):3160(2020) [pubmed]

submitter keyword: primary couplling, secondary coupling,GPCR, G protein, HDX-MS

Ka Young Chung
contact affiliation	School of Pharmacy, Sungkyunkwan University, South Korea
contact email	kychung2@skku.edu
lab head
Hee Ryung Kim
contact affiliation	Hospital for Sick Children Research Institute
contact email	heeryung.jan.kim@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019367

PRIDE project URI

• PRIDE
  1. PXD019367
     1. Label: PRIDE project
     2. Name: Structural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family