PXD019355 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metaproteomic Profiling of Functional Changes Driven by Gut Fungal Colonization |
Description | Gut microbiome modulates the host immune development, yet the functional contribution of gut fungi remains elusive. We previously showed that mice colonized only with fungi displayed allergic features and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed proteomic analyses of feces and small intestine of gnotobiotic mice colonized with either bacteria, fungi, or both. Comparison of fecal metaproteomic profiles between mouse groups yielded broad changes in the relative levels of bacterial, fungal and mouse proteins. Many of the detected fungal proteins have been previously reported as a part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the small intestine impacted essential cellular pathways, including lipid metabolism and apoptosis. The results show how fungal colonization impacts the host proteome and suggest an influence on the host final cellular phenotype. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:34:19.898.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Veronika Pettersen |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-24 22:44:52 | ID requested | |
1 | 2022-02-28 12:32:27 | announced | |
⏵ 2 | 2024-10-22 05:34:20 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1186/s42523-022-00163-2; |
Pettersen VK, Dufour A, Arrieta MC, Metaproteomic profiling of fungal gut colonization in gnotobiotic mice. Anim Microbiome, 4(1):14(2022) [pubmed] |
Keyword List
submitter keyword: gut fungi,early-life gut microbiome, LFQ and TMT proteomics, gnotobiotic mice |
Contact List
Marie-Claire Arrieta |
contact affiliation | Depts. of Physiology and Pharmacology & Pediatrics Cumming School of Medicine University of Calgary, Canada |
contact email | marie.arrieta@ucalgary.ca |
lab head | |
Veronika Pettersen |
contact affiliation | Department of Pediatrics, University Hospital of North Norway, Tromsø, Norway; Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø Norway |
contact email | veronika.k.pettersen@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD019355 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019355
- Label: PRIDE project
- Name: Metaproteomic Profiling of Functional Changes Driven by Gut Fungal Colonization