PXD019355

PXD019355 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Metaproteomic Profiling of Functional Changes Driven by Gut Fungal Colonization
Description	Gut microbiome modulates the host immune development, yet the functional contribution of gut fungi remains elusive. We previously showed that mice colonized only with fungi displayed allergic features and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed proteomic analyses of feces and small intestine of gnotobiotic mice colonized with either bacteria, fungi, or both. Comparison of fecal metaproteomic profiles between mouse groups yielded broad changes in the relative levels of bacterial, fungal and mouse proteins. Many of the detected fungal proteins have been previously reported as a part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the small intestine impacted essential cellular pathways, including lipid metabolism and apoptosis. The results show how fungal colonization impacts the host proteome and suggest an influence on the host final cellular phenotype.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:19.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronika Pettersen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-24 22:44:52	ID requested
1	2022-02-28 12:32:27	announced
⏵ 2	2024-10-22 05:34:20	announced	2024-10-22: Updated project metadata.

Publication List

10.1186/s42523-022-00163-2;
Pettersen VK, Dufour A, Arrieta MC, Metaproteomic profiling of fungal gut colonization in gnotobiotic mice. Anim Microbiome, 4(1):14(2022) [pubmed]

10.1186/s42523-022-00163-2;

Pettersen VK, Dufour A, Arrieta MC, Metaproteomic profiling of fungal gut colonization in gnotobiotic mice. Anim Microbiome, 4(1):14(2022) [pubmed]

Keyword List

submitter keyword: gut fungi,early-life gut microbiome, LFQ and TMT proteomics, gnotobiotic mice

submitter keyword: gut fungi,early-life gut microbiome, LFQ and TMT proteomics, gnotobiotic mice

Contact List

Marie-Claire Arrieta
contact affiliation	Depts. of Physiology and Pharmacology & Pediatrics Cumming School of Medicine University of Calgary, Canada
contact email	marie.arrieta@ucalgary.ca
lab head
Veronika Pettersen
contact affiliation	Department of Pediatrics, University Hospital of North Norway, Tromsø, Norway; Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø Norway
contact email	veronika.k.pettersen@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD019355
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD019355

PRIDE project URI

Repository Record List

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