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PXD019355

PXD019355 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMetaproteomic Profiling of Functional Changes Driven by Gut Fungal Colonization
DescriptionGut microbiome modulates the host immune development, yet the functional contribution of gut fungi remains elusive. We previously showed that mice colonized only with fungi displayed allergic features and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed proteomic analyses of feces and small intestine of gnotobiotic mice colonized with either bacteria, fungi, or both. Comparison of fecal metaproteomic profiles between mouse groups yielded broad changes in the relative levels of bacterial, fungal and mouse proteins. Many of the detected fungal proteins have been previously reported as a part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the small intestine impacted essential cellular pathways, including lipid metabolism and apoptosis. The results show how fungal colonization impacts the host proteome and suggest an influence on the host final cellular phenotype.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:34:19.898.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVeronika Pettersen
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-24 22:44:52ID requested
12022-02-28 12:32:27announced
22024-10-22 05:34:20announced2024-10-22: Updated project metadata.
Publication List
10.1186/s42523-022-00163-2;
Pettersen VK, Dufour A, Arrieta MC, Metaproteomic profiling of fungal gut colonization in gnotobiotic mice. Anim Microbiome, 4(1):14(2022) [pubmed]
Keyword List
submitter keyword: gut fungi,early-life gut microbiome, LFQ and TMT proteomics, gnotobiotic mice
Contact List
Marie-Claire Arrieta
contact affiliationDepts. of Physiology and Pharmacology & Pediatrics Cumming School of Medicine University of Calgary, Canada
contact emailmarie.arrieta@ucalgary.ca
lab head
Veronika Pettersen
contact affiliationDepartment of Pediatrics, University Hospital of North Norway, Tromsø, Norway; Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø Norway
contact emailveronika.k.pettersen@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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