PXD019354

PXD019354 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterisation of host-pathogen interactions during bovine trophoblast cell line infection by Neospora caninum
Description	Despite the importance of bovine neosporosis, important knowledge gaps remain concerning pathogenic mechanisms of N. caninum. The infection of the placenta is a crucial event in the pathogenesis of the disease, however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3, involving proliferative capacity and transcriptome host cell modulation. Herein, we use the same model of infection to study the interaction of Neospora with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analyzed the proteome differences between high (Nc-Spain7)- and low (Nc-Spain1H)- virulence isolates. The results showed mitochondrial processes and metabolism as the main point of Neospora-host interactions. Interestingly, Nc-Spain1H infection showed the highest level of influence on host cell proteome in contrast to Nc-Spain7 infection. These results confirm Nc-Spain7 capacities to evade host cell response and establish infection more effectively.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:30.462.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Neospora Caninum (strain Liverpool); NCBI TaxID: 572307;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-24 22:41:33	ID requested
1	2021-09-08 19:11:02	announced
⏵ 2	2024-10-22 05:27:38	announced	2024-10-22: Updated project metadata.

10.3390/pathogens9090749;

Regidor-Cerrillo J, Xia D, Jim, é, nez-Pelayo L, Garc, í, a-S, á, nchez M, Collantes-Fern, á, ndez E, Randle N, Wastling J, Ortega-Mora LM, Horcajo P, . Pathogens, 9(9):(2020) [pubmed]

submitter keyword: bovine trophoblast cell line host-parasite interaction,Neospora caninum, isolate virulence, proteome

Dr Dong Xia
contact affiliation	The Royal Veterinary College Royal College Street London NW1 0TU United Kingdom
contact email	dxia@rvc.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019354

PRIDE project URI

• PRIDE
  1. PXD019354
     1. Label: PRIDE project
     2. Name: Proteomic characterisation of host-pathogen interactions during bovine trophoblast cell line infection by Neospora caninum