PXD019348

PXD019348 is an original dataset announced via ProteomeXchange.

Title	Proteome-Wide Effects of Naphthalene-Derived Secondary Organic Aerosol in BEAS-2B Cells Are Caused by Short-Lived Unsaturated Carbonyls
Description	Exposure to air pollution causes adverse health outcomes but the toxicity mechanisms remain unclear. Here, we investigated the dynamic toxicities of naphthalene-derived secondary organic aerosol (NSOA) in human bronchial epithelial cell line (BEAS-2B) and identified the chemical components responsible for toxicities. The chemical composition of NSOA was found to vary with six simulated atmospheric aging conditions (C1-C6), as characterized by high-resolution mass spectrometry and ion mobility mass spectrometry. Global proteome profiling reveals dynamic evolution in toxicity: stronger proteome-wide impacts were detected in fresh NSOA, but the effects declined along with atmospheric aging. While Nrf2-regulated proteins (e.g., NQO1) were significantly upregulated, the majority (78-97%) of proteins from inflammation and other pathways were down-regulated by NSOA exposure (e.g., Rho GTPases). This pattern is distinct from the reactive oxygen species (ROS) mediated toxicity pathway, and an alternative cysteine-reaction pathway was revealed by the decreased abundance of proteins (e.g., MT1X) prone to post-translational thiol modification. This pathway was further validated by observing decreased Nrf2 response in reporter cells, after pre-incubating NSOA with cysteine. Ethynyl-naphthalene probe was employed to confirm the alkylation of cellular proteome thiols on the proteome-wide level by fresh NSOA via in-gel fluorescence imaging. Nontarget analysis identified several unsaturated carbonyls, including naphthoquinones and hydroxylated naphthoquinones, as the toxic components responsible for cysteine reactivity. Our study provides insights into the dynamic toxicities of NSOA during atmospheric aging and identifies short-lived unsaturated carbonyls as the predominant toxic components at the post-translational level.
HostingRepository	PRIDE
AnnounceDate	2020-09-29
AnnouncementXML	Submission_2020-09-28_22:38:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiajun Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-22 03:35:52	ID requested
⏵ 1	2020-09-28 22:38:19	announced

Dataset with its publication pending

submitter keyword: BEAS-2B,NSOA,Orbitrap,DDA,maxquant

Hui Peng
contact affiliation	Department of Chemistry, University of Toronto, Toronto, ON, Canada, M5S 3H6
contact email	hui.peng@utoronto.ca
lab head
Jiajun Han
contact affiliation	University of Toronto
contact email	hanjiajun1990216@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019348
     1. Label: PRIDE project
     2. Name: Proteome-Wide Effects of Naphthalene-Derived Secondary Organic Aerosol in BEAS-2B Cells Are Caused by Short-Lived Unsaturated Carbonyls