PXD019343

PXD019343 is an original dataset announced via ProteomeXchange.

Title	A multi-omics approach unravels new toxins with potential antimicrobial, antiviral and antitumoral activities in the venom of Acanthoscurria rondoniae
Description	The Araneae order is considered one of the most successful group among venomous animals in the world. An important factor for this success is the production of venoms, a refined biological fluid rich in proteins, short peptides and cysteine-rich peptides (CRPs). These toxins may present pharmacologically relevant biological actions, as antimicrobial, antiviral and anticancer activities, for instance. Therefore, there is an increasing interest in the exploration of venom toxins for therapeutic reasons, such as drug development. However, the process of peptide sequencing and mainly the evaluation of potential biological activities of these peptides is laborious, considering the low yield of venom extraction and the high variability of toxins present in spider venoms. Here we show a robust methodology for identification, sequencing, and initial screening of potential bioactive peptides found in the venom of Acanthoscurria rondoniae. This methodology consists in a multi-omics approach involving proteomics, peptidomics and transcriptomics analyses allied to in silico predictions of antibacterial, antifungal, antiviral and anticancer activities. Through the application of this strategy, a total of 92,889 venom gland transcripts were assembled and 84 novel toxins were identified at the protein level, including 7 short peptides and 10 fully sequenced CRPs (belonging to 7 toxin families). In silico analysis revealed that 7 CRPs families have potential antimicrobial or antiviral activities, while 2 CRPs and four short peptides are potentially anticancer. Taken together, our results demonstrate an effective multi-omics strategy for the discovery of new toxins and in silico screening of potential bioactivities. This strategy may be useful in toxin discovery, as well as in the screening of activities for the vast diversity of molecules produced by venomous animals.
HostingRepository	PRIDE
AnnounceDate	2020-07-20
AnnouncementXML	Submission_2020-08-11_22:47:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Acanthoscurria rondoniae; NCBI TaxID: 1211104;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-22 01:37:28	ID requested
1	2020-07-19 21:57:33	announced
⏵ 2	2020-08-11 22:47:20	announced	2020-08-12: Updated publication reference for PubMed record(s): 32774304.

C, â, mara GA, Nishiyama-Jr MY, Kitano ES, Oliveira UC, da Silva PI, Junqueira-de-Azevedo IL, Tashima AK, . Front Pharmacol, 11():1075(2020) [pubmed]

submitter keyword: Acanthoscurria rondoniae, cysteine-rich peptides, peptidomics, proteomics, multi-omics, 33 antimicrobial peptides, antiviral peptides, antitumoral peptides

Alexandre Keiji Tashima
contact affiliation	Department of Biochemistry, Federal University of Sao Paulo
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019343

PRIDE project URI

• PRIDE
  1. PXD019343
     1. Label: PRIDE project
     2. Name: A multi-omics approach unravels new toxins with potential antimicrobial, antiviral and antitumoral activities in the venom of Acanthoscurria rondoniae