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PXD019328

PXD019328 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranslation Error Clusters Induced by Aminoglycoside Antibiotics
DescriptionAminoglycoside antibiotics (AGAs) target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal AGAs induce not only single translation errors, but also clusters of errors in full-length proteins in vivo with as much as four amino acid substitutions in a row. The downstream errors in a cluster are much more frequent than the first error (0.4 vs. 10-3, respectively) and independent of the intracellular AGA concentration. The prevalence, length, and composition of error clusters depends not only on the misreading propensity of a given AGA, but also on its ability to inhibit ribosome translocation along the mRNA. Error clusters constitute a new class of misreading events in vivo that may constitute the predominant source of proteotoxic stress at low AGA concentration, which is particularly important for the autocatalytic uptake of the drugs.
HostingRepositoryPanoramaPublic
AnnounceDate2021-04-06
AnnouncementXMLSubmission_2021-04-06_06:33:34.018.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterIngo Wohlgemuth
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF-X; Q Exactive Plus; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-21 13:21:37ID requested
12021-04-06 06:33:34announced
Publication List
Wohlgemuth I, Garofalo R, Samatova E, Günenç AN, Lenz C, Urlaub H, Rodnina MV, Translation error clusters induced by aminoglycoside antibiotics. Nat Commun, 12(1):1830(2021) [pubmed]
Keyword List
submitter keyword: aminoglycosides, ribosome, miscoding, misreading, antibiotics
Contact List
Ingo Wohlgemuth
contact affiliationMax-Planck-Institute for Biophysical Chemistry, Department for Physical Biochemistry, Am Fassberg 11, 37077 Goettingen, Germany
contact emailingo.wohlgemuth@mpibpc.mpg.de
lab head
Ingo Wohlgemuth
contact affiliationMax-Planck-Institute for Biophysical Chemistry, Department for Physical Biochemistry, Am Fassberg 11, 37077 Goettingen, Germany
contact emailingo.wohlgemuth@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI