PXD019328

PXD019328 is an original dataset announced via ProteomeXchange.

Title	Translation Error Clusters Induced by Aminoglycoside Antibiotics
Description	Aminoglycoside antibiotics (AGAs) target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal AGAs induce not only single translation errors, but also clusters of errors in full-length proteins in vivo with as much as four amino acid substitutions in a row. The downstream errors in a cluster are much more frequent than the first error (0.4 vs. 10-3, respectively) and independent of the intracellular AGA concentration. The prevalence, length, and composition of error clusters depends not only on the misreading propensity of a given AGA, but also on its ability to inhibit ribosome translocation along the mRNA. Error clusters constitute a new class of misreading events in vivo that may constitute the predominant source of proteotoxic stress at low AGA concentration, which is particularly important for the autocatalytic uptake of the drugs.
HostingRepository	PanoramaPublic
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2021-04-06_06:33:34.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Ingo Wohlgemuth
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF-X; Q Exactive Plus; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-21 13:21:37	ID requested
⏵ 1	2021-04-06 06:33:34	announced

Wohlgemuth I, Garofalo R, Samatova E, G, ü, nen, ç AN, Lenz C, Urlaub H, Rodnina MV, Translation error clusters induced by aminoglycoside antibiotics. Nat Commun, 12(1):1830(2021) [pubmed]

submitter keyword: aminoglycosides, ribosome, miscoding, misreading, antibiotics

Ingo Wohlgemuth
contact affiliation	Max-Planck-Institute for Biophysical Chemistry, Department for Physical Biochemistry, Am Fassberg 11, 37077 Goettingen, Germany
contact email	ingo.wohlgemuth@mpibpc.mpg.de
lab head
Ingo Wohlgemuth
contact affiliation	Max-Planck-Institute for Biophysical Chemistry, Department for Physical Biochemistry, Am Fassberg 11, 37077 Goettingen, Germany
contact email	ingo.wohlgemuth@mpibpc.mpg.de
dataset submitter

Panorama Public dataset URI