PXD019317

PXD019317 is an original dataset announced via ProteomeXchange.

Title	ozone-derived oxisterols impair lung macrophage phagocytosis via adduction of phagocytosis receptors
Description	Inhalation of the amibient air polution ozone causes lung inflammation and can suppress host defense mechanisms, including impairing macrophage phagocytosis. Ozone reacts with cholesterol in the lung to form oxysterols, like secosterol A and secosterol B, which can form covalent adducts on cellular proteins. How oxysterol-protein adduction modifies the function of lung macrophages is unknown. Herein, we used a preoteomic screen to identify lung macrophage proteins that fomr adducts with ozone-derived oxysterols. Analysis show that the phagocytic receptor CD206 and CD64 formed adducts with secosterol A. Adduction of these receptors with ozone-derived oxysterols impaired ligand binding and corresponded with reduced macrophage phagocytosis. This work suggests a novle mechanism for the suppression of macrophage phagocytosis following ozone exposure through the generation of oxysterols and the formation of oxysterol-protein adducts on phagocytic receptors.
HostingRepository	PRIDE
AnnounceDate	2020-07-18
AnnouncementXML	Submission_2020-07-26_23:00:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019317
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hye-Young Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	unknown modification; Oxidation; Carbamidomethyl; iodoacetamide derivatized residue; Gln->pyro-Glu
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-20 17:41:28	ID requested
1	2020-07-17 17:48:11	announced
⏵ 2	2020-07-26 23:00:01	announced	2020-07-27: Updated publication reference for PubMed record(s): 32690608.

Duffney PF, Kim HH, Porter NA, Jaspers I, Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors. J Biol Chem, 295(36):12727-12738(2020) [pubmed]

submitter keyword: human, lung macrophage, bronchoalveolar lavage, CD206, click, secosterol A/B, ozone

Ilona, Jaspers
contact affiliation	University of North Carolina, Curriculum in Toxicology and Environmental Medicine, Chapel Hill, NC, USA
contact email	ilona_jaspers@med.unc.edu
lab head
Hye-Young Kim
contact affiliation	Vanderbilt University
contact email	hye-young.h.kim@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019317
     1. Label: PRIDE project
     2. Name: ozone-derived oxisterols impair lung macrophage phagocytosis via adduction of phagocytosis receptors