PXD019311 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improved Monoisotopic Mass Estimation for Deeper Proteome Coverage |
Description | Accurate assignment of monoisotopic peaks is essential for the identification of peptides in bottom-up proteomics. Misassignment or inaccurate attribution of peptidic ions leads to lower sensitivity and fewer total peptide identifications. In the present work we present a performant, open-source, cross-platform algorithm, Monocle, for the rapid reassignment of instrument assigned precursor peaks to monoisotopic peptide assignments. We demonstrate that the present algorithm can be integrated into many common proteomics pipelines and provides rapid conversion from multiple data source types. Finally, we show that our monoisotopic peak assignment results in up to a two-fold increase in total peptide identifications compared to analyses lacking monoisotopic correction and a 44% improvement over previous monoisotopic peak correction algorithms. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-17 |
AnnouncementXML | Submission_2020-11-17_09:07:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiaming Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-20 17:22:10 | ID requested | |
⏵ 1 | 2020-11-17 09:07:30 | announced | |
Publication List
Rad R, Li J, Mintseris J, O'Connell J, Gygi SP, Schweppe DK, Improved Monoisotopic Mass Estimation for Deeper Proteome Coverage. J Proteome Res, 20(1):591-598(2021) [pubmed] |
Keyword List
submitter keyword: Monoisotopic peak, TMT, multiplex proteomics, bottom-up proteomics, FAIMS, APD |
Contact List
Steven Gygi |
contact affiliation | Harvard Medical School |
contact email | steven_gygi@hms.harvard.edu |
lab head | |
Jiaming Li |
contact affiliation | Harvard Medical School |
contact email | jiaming_li@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019311 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019311
- Label: PRIDE project
- Name: Improved Monoisotopic Mass Estimation for Deeper Proteome Coverage