PXD019301

PXD019301 is an original dataset announced via ProteomeXchange.

Title	Appressorial proteomics analysis of the wild-type and GAG biosynthetic mutant of Metarhizium robertsii
Description	Exopolysaccharide galactosaminogalactan (GAG) is a fungal cell wall component composed of α-1,4 linked galactose, N-acetyl galactosamine and galactosamine, which has been demonstrated in Aspergillus fumigatus in association with fungal adhesion, biofilm formation and virulence. The gene cluster responsible for GAG biosynthesis has only been characterized in Aspergillus fungi. We found that the highly conserved gene cluster for GAG biosynthesis is also present in the insect pathogenic fungi Metarhizium species. Functional investigations in M. robertsii revealed that GAG is only produced on fungal cell wall during fungal germination, filamentation and the formation of the infection structure appressoria. Gene deletions revealed that, relative to the wild-type (WT), the appressorial mucilage production was abolished in the null mutant of M. robertsii. Since multiple enzymes are produced in appressorial mucilages, appressorial samples of the WT and mutant formed on cicada wings were collected and subjected to iTRAQ comparative proteomic analysis. We found that different protein families were up- or down-regulated in the null mutant when compared with the WT.
HostingRepository	PRIDE
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2021-06-07_04:28:24.650.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanzhen Zhao
SpeciesList	scientific name: Metarhizium robertsii; NCBI TaxID: 568076;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-19 23:00:38	ID requested
⏵ 1	2021-06-07 04:28:25	announced

Dataset with its publication pending

submitter keyword: GAG,Metarhizium robertsii,iTRAQ

Chengshu Wang
contact affiliation	CAS Center for Excellence in Molecular Plant Science(CEMPS)/Institute of Plant Physiology and Ecology, Chinese Academy of Sciences (CAS)
contact email	cswang@sibs.ac.cn
lab head
Yanzhen Zhao
contact affiliation	shanghai
contact email	449096780@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019301
     1. Label: PRIDE project
     2. Name: Appressorial proteomics analysis of the wild-type and GAG biosynthetic mutant of Metarhizium robertsii