PXD019299
PXD019299 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cardioprotection via Endothelial Extracellular Vesicles in a Human Heart-on-Chip Ischemia-Reperfusion-Injury Model |
Description | We have mapped and analyzed the protein content of extracellular vesicles isolated from HUVECs (EEVs). Several groups of cardioprotective proteins were detected, as well as proteins related to various metabolic processes and response to stress. We have tested the effect of these EEVs on human stem cell- derived cardiomyocytes (hCMS) experiencing ischemia reperfusion injury (IRI). We have demonstrated the EEV treatment reduced cell death following IRI, and preserved contractile function. The effect depended on EEVs internalization in hCMS. We have compared protein expression profiles of hCMs treated and untreated with EEVs, under normal conditions and following IRI. We show that EEVs induce changes in the protein expression profiles of normal hCMs. The changes are related to various transcriptional and metabolic processes and localized in mainly in the nucleus and mitochondrion. After induction of IRI the differences between the EEV-treated and untreated groups are enhanced with a larger amount of differentially expressed proteins, with the most significant changes in metabolic processes and binding activity. |
HostingRepository | MassIVE |
AnnounceDate | 2020-10-14 |
AnnouncementXML | Submission_2020-10-14_08:16:57.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Bogdan Budnik |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation; TMT6plex; TMT; Deamidated; Phospho |
Instrument | LTQ Orbitrap Elite; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-19 12:30:05 | ID requested | |
⏵ 1 | 2020-10-14 08:16:57 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Human, HUVEC, EVs, human cardiomyocytes (hCMs), EEVs, IRI |
Contact List
Kevin Kit Parker | |
---|---|
contact affiliation | Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University |
contact email | kkparker@seas.harvard.edu |
lab head | |
Bogdan Budnik | |
contact affiliation | Harvard University |
contact email | bbudnik@mcb.harvard.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085448/ |