PXD019299

PXD019299 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cardioprotection via Endothelial Extracellular Vesicles in a Human Heart-on-Chip Ischemia-Reperfusion-Injury Model
Description	We have mapped and analyzed the protein content of extracellular vesicles isolated from HUVECs (EEVs). Several groups of cardioprotective proteins were detected, as well as proteins related to various metabolic processes and response to stress. We have tested the effect of these EEVs on human stem cell- derived cardiomyocytes (hCMS) experiencing ischemia reperfusion injury (IRI). We have demonstrated the EEV treatment reduced cell death following IRI, and preserved contractile function. The effect depended on EEVs internalization in hCMS. We have compared protein expression profiles of hCMs treated and untreated with EEVs, under normal conditions and following IRI. We show that EEVs induce changes in the protein expression profiles of normal hCMs. The changes are related to various transcriptional and metabolic processes and localized in mainly in the nucleus and mitochondrion. After induction of IRI the differences between the EEV-treated and untreated groups are enhanced with a larger amount of differentially expressed proteins, with the most significant changes in metabolic processes and binding activity.
HostingRepository	MassIVE
AnnounceDate	2020-10-14
AnnouncementXML	Submission_2020-10-14_08:16:57.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bogdan Budnik
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; TMT6plex; TMT; Deamidated; Phospho
Instrument	LTQ Orbitrap Elite; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-19 12:30:05	ID requested
⏵ 1	2020-10-14 08:16:57	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Human, HUVEC, EVs, human cardiomyocytes (hCMs), EEVs, IRI

submitter keyword: Human, HUVEC, EVs, human cardiomyocytes (hCMs), EEVs, IRI

Contact List

Kevin Kit Parker
contact affiliation	Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University
contact email	kkparker@seas.harvard.edu
lab head
Bogdan Budnik
contact affiliation	Harvard University
contact email	bbudnik@mcb.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085448/