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PXD019299

PXD019299 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCardioprotection via Endothelial Extracellular Vesicles in a Human Heart-on-Chip Ischemia-Reperfusion-Injury Model
DescriptionWe have mapped and analyzed the protein content of extracellular vesicles isolated from HUVECs (EEVs). Several groups of cardioprotective proteins were detected, as well as proteins related to various metabolic processes and response to stress. We have tested the effect of these EEVs on human stem cell- derived cardiomyocytes (hCMS) experiencing ischemia reperfusion injury (IRI). We have demonstrated the EEV treatment reduced cell death following IRI, and preserved contractile function. The effect depended on EEVs internalization in hCMS. We have compared protein expression profiles of hCMs treated and untreated with EEVs, under normal conditions and following IRI. We show that EEVs induce changes in the protein expression profiles of normal hCMs. The changes are related to various transcriptional and metabolic processes and localized in mainly in the nucleus and mitochondrion. After induction of IRI the differences between the EEV-treated and untreated groups are enhanced with a larger amount of differentially expressed proteins, with the most significant changes in metabolic processes and binding activity.
HostingRepositoryMassIVE
AnnounceDate2020-10-14
AnnouncementXMLSubmission_2020-10-14_08:16:57.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBogdan Budnik
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; TMT6plex; TMT; Deamidated; Phospho
InstrumentLTQ Orbitrap Elite; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-19 12:30:05ID requested
12020-10-14 08:16:57announced
Publication List
no publication
Keyword List
submitter keyword: Human, HUVEC, EVs, human cardiomyocytes (hCMs), EEVs, IRI
Contact List
Kevin Kit Parker
contact affiliationHarvard John A. Paulson School of Engineering and Applied Sciences, Harvard University
contact emailkkparker@seas.harvard.edu
lab head
Bogdan Budnik
contact affiliationHarvard University
contact emailbbudnik@mcb.harvard.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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