PXD019297
PXD019297 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The Greatwall kinase safeguards the genome integrity by balancing the kinome activity in mitosis. |
| Description | Mastl is commonly overexpressed in cancer, appearing as an alternative therapeutic anticancer target. Loss of Mastl induces multiple chromosomal mitotic errors that lead to the accumulation of micronuclei and multilobulated cells with polyploidy. Our detailed analyses display that loss of Mastl quickly lead to chromosomes breakage and abnormalities impairing correct segregation. Phosphoproteomic data of mouse embryonic fibroblasts revealed defects in kinetochores, perichromosomes, and centrosomes but also on RNA binding proteins and double strand DNA damage repair.In our study, Rad51ap1, a well-known homologous recombination regulator, appeared to be effectively phosphorylated by Nek2 and CDK1, but also efficiently depshosphorylated by PP2A/B55. Taken together, these results suggest that Mastl loss induces a multitude of alteration even in noncancerous cells that lead to the disruption of DNA damage repair triggering chromosomes breakage and in consequence, creates an accumulative disequilibrium in the phosphoproteome. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-10-15 |
| AnnouncementXML | Submission_2022-09-18_03:02:57.697.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Radoslaw Sobota |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-threonine; O-phospho-L-serine; O4'-phospho-L-tyrosine; unknown modification |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-05-19 06:48:12 | ID requested | |
| 1 | 2020-10-15 01:21:50 | announced | |
| 2 | 2021-02-04 16:35:43 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 3 | 2022-09-18 03:02:58 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Bisteau X, Lee J, Srinivas V, Lee JHS, Niska-Blakie J, Tan G, Yap SYX, Hom KW, Wong CK, Chae J, Wang LC, Kim J, Rancati G, Sobota RM, Tan CSH, Kaldis P, The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis. Oncogene, 39(44):6816-6840(2020) [pubmed] |
Keyword List
| submitter keyword: MASTL, mitosis, phosphoproteomics, TMT |
Contact List
| Xavier Bisteau | |
|---|---|
| lab head | |
| Radoslaw Sobota | |
| contact affiliation | IMCB A-STAR Singapore |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000837/ |
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