PXD019291

PXD019291 is an original dataset announced via ProteomeXchange.

Title	ABNORMAL POLLEN 1 (AP1) encodes a legume-type lectin receptor-like kinase and is involved in starch accumulation during rice pollen maturation
Description	Starch accumulation is the key progress for the maturity of rice pollen grains. However, the regulatory mechanism underlying which remains less understood. Here, we isolated a rice male-sterile mutant ap1, which produces non-viable pollen grains with defective starch accumulation. Functional analysis revealed that AP1 encodes an active L-type lectin receptor-like kinase (L-LecRLK). AP1 is located on plasma membrane and its transcript is highly accumulated in pollen during the starch synthesis phase. RNA-seq analysis suggests that the mutation of AP1 significantly altered the expression of numerous genes involved in starch and sucrose metabolic pathway. Phosphoproteomic profiling revealed that the phosphorylation levels of several proteins within this pathway were significantly downregulated in the mutant. Among which, a rice UDP-glucose pyrophosphorylase (OsUGP2), which roles in pollen starch accumulation, was identified. Further protein–protein interaction assays suggest that OsUGP2 is a feasible target of AP1 to regulate pollen starch accumulation. Our findings thus revealed a novel role of L-LecRLK in controlling pollen maturity via modulating starch metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:41.731.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhiyuan he
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-19 01:49:51	ID requested
1	2021-03-06 06:24:06	announced
⏵ 2	2024-10-22 05:19:42	announced	2024-10-22: Updated project metadata.

He Z, Zou T, Xiao Q, Yuan G, Liu M, Tao Y, Zhou D, Zhang X, Deng Q, Wang S, Zheng A, Zhu J, Liang Y, Yu X, Wang A, Liu H, Wang L, Li P, Li S, An L-type lectin receptor-like kinase promotes starch accumulation during rice pollen maturation. Development, 148(6):(2021) [pubmed]

10.1242/dev.196378;

submitter keyword: Rice, Protein interaction,Pollen, Starch, AP1, Phosphorylation

Zhiyuan he
contact affiliation	Sichuan Agricultural University
contact email	hezy1205@163.com
lab head
zhiyuan he
contact affiliation	Sichuan Agricultural University
contact email	1615591035@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019291
     1. Label: PRIDE project
     2. Name: ABNORMAL POLLEN 1 (AP1) encodes a legume-type lectin receptor-like kinase and is involved in starch accumulation during rice pollen maturation