PXD019290

PXD019290 is an original dataset announced via ProteomeXchange.

Title	Chemical Penetration Enhancers Increase Hydrogen Peroxide Uptake in C. elegans for In Vivo Fast Photochemical Oxidation of Proteins
Description	Fast photochemical oxidation of proteins (FPOP) is a hydroxyl radical protein footprinting method that covalently labels solvent accessible amino acids by photolysis of hydrogen peroxide. Recently, we expanded the use of FPOP for in vivo (IV-FPOP) covalent labeling in C. elegans. In initial IV-FPOP studies, 545 proteins were oxidatively modified in all body systems within the worm. Here, with the use of chemical penetration enhancers (CPEs), we increased the number of modified proteins as well as the number of modifications per protein to gain increased structural information. CPEs aid in the delivery of hydrogen peroxide inside C. elegans by disturbing the highly order lipid bilayer of the worm cuticle without affecting worm viability. IV-FPOP experiments performed using the CPE azone showed an increase in oxidatively modified proteins and peptides. This increase correlated with greater hydrogen peroxide uptake by C. elegans quantified using a chemical fluorophore demonstrating the efficacy of using CPEs with IV-FPOP.
HostingRepository	PRIDE
AnnounceDate	2020-06-11
AnnouncementXML	Submission_2020-06-11_15:18:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019290
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessica Espino
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-19 01:10:05	ID requested
⏵ 1	2020-06-11 15:18:28	announced

Espino JA, Zhang Z, Jones LM, Fast Photochemical Oxidation of Proteins. J Proteome Res, 19(9):3708-3715(2020) [pubmed]

submitter keyword: in vivo, protein footprinting, FPOP, C. elegans, chemical penetration enhancers

Lisa Jones
contact affiliation	Department of Pharmaceutical Sciences, University of Maryland, Baltimore, Maryland 21202, United States
contact email	ljones@rx.umaryland.edu
lab head
Jessica Espino
contact affiliation	Department of Pharmaceutical Sciences, University of Maryland, Baltimore, Maryland 21202, United States
contact email	jespino@umaryland.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019290
     1. Label: PRIDE project
     2. Name: Chemical Penetration Enhancers Increase Hydrogen Peroxide Uptake in C. elegans for In Vivo Fast Photochemical Oxidation of Proteins