PXD019289

PXD019289 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype
Description	The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. A total of 1,976 proteins have been identified using public databases. One hundred ninety six proteins were found significantly differentially expressed; results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. This comparison selected thirty six proteins as discriminant biomarkers among A. simplex, A. pegreffii and their hybrid genotype; eighteen of these biomarkers, encoded by nine loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12 and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen.
HostingRepository	PRIDE
AnnounceDate	2020-08-17
AnnouncementXML	Submission_2020-08-16_22:54:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019289
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Anisakis simplex complex; NCBI TaxID: 644710;
ModificationList	iTRAQ4plex; Methylthio; Glu->pyro-Glu; Oxidation; Acetyl; Gln->pyro-Glu
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-19 01:06:44	ID requested
⏵ 1	2020-08-16 22:54:36	announced

C Arcos S, Robertson L, Ciordia S, S, á, nchez-Alonso I, Careche M, Carballeda-Sanguiao N, Gonzalez-Mu, ñ, oz M, Navas A, , and Their Hybrid Genotype. Genes (Basel), 11(8):(2020) [pubmed]

submitter keyword: Anisakis simplex, allergens, oesphageal glands, proteomics, excretion, secretion

Alfonso Navas
contact affiliation	Museo Nacional de Ciencias Naturales. Dpto Biodiversidad y Biologia Evolutiva (CSIC)
contact email	anavas@mncn.csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019289
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype