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PXD019275

PXD019275 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCryo-EM structures of holo condensin reveal a subunit flip-flop mechanism
DescriptionThis data is from BS3 crosslinked condensin tetramer How protein complexes of the SMC family fold DNA into the large loops that are fundamental for the 3D organization of genomes is a central unresolved question of chromosome biology. We used electron cryomicroscopy to investigate the reaction cycle of the SMC complex condensin, which is a key determinant of chromosome morphology and behavior during mitosis. Our structures of the Saccharomyces cerevisiae condensin holo complex at different functional stages suggest that ATP binding induces the transition from a folded-rod SMC conformation into an open architecture and triggers the exchange of the two HEAT-repeat subunits at the SMC ATPase head domains. We propose that these steps result in the interconversion of DNA binding sites in the catalytic core that form the basis of the DNA translocation and loop-extrusion activities of condensin.
HostingRepositoryPRIDE
AnnounceDate2020-07-28
AnnouncementXMLSubmission_2020-07-27_23:53:03.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancis O'Reilly
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-18 17:06:14ID requested
12020-07-27 23:53:05announced
Publication List
Lee BG, Merkel F, Allegretti M, Hassler M, Cawood C, Lecomte L, O'Reilly FJ, Sinn LR, Gutierrez-Escribano P, Kschonsak M, Bravo S, Nakane T, Rappsilber J, Aragon L, Beck M, L, ö, we J, Haering CH, Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism. Nat Struct Mol Biol, 27(8):743-751(2020) [pubmed]
Keyword List
submitter keyword: condensin, tetramer, crosslinking, BS3
Contact List
Juri Rappsilber
contact affiliationBioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK.
contact emailjuri.rappsilber@tu-berlin.de
lab head
Francis O'Reilly
contact affiliationTU Berlin
contact emailoreilly@tu-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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