PXD019268

PXD019268 is an original dataset announced via ProteomeXchange.

Title	Rubisco acetylation stoichiometry in Arabidopsis Thaliana
Description	Multiple studies have shown Rubisco to be subject to Lys-acetylation at various residues; however, conflicting reports exist about the biological significance of these post-translational modifications. One aspect of the Lys-acetylation that has not been addressed in plants generally, or with Rubisco specifically, is the stoichiometry at which these Lys-acetylation events occur. As a method to ascertain which Lys-acetylation sites on Arabidopsis Rubisco might be of regulatory importance, we purified Rubisco from leaves in both the day and night-time and performed independent mass-spectrometry based methods to determine the stoichiometry of Rubisco Lys-acetylation events.
HostingRepository	PRIDE
AnnounceDate	2020-09-28
AnnouncementXML	Submission_2020-09-27_23:38:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brendan O'Leary
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 16:57:36	ID requested
⏵ 1	2020-09-27 23:38:12	announced

O'Leary BM, Scafaro AP, Fenske R, Duncan O, Str, ö, her E, Petereit J, Millar AH, Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme function. Biochem J, 477(19):3885-3896(2020) [pubmed]

submitter keyword: Arabidopsis, Lys-acetylation, Rubisco

Harvey Millar
contact affiliation	Centre of Excellence in Plant Energy Biology, Department of Molecular Sciences, University of Western Australia
contact email	havey.millar@uwa.edu.au
lab head
Brendan O'Leary
contact affiliation	University of Western Australia
contact email	brendan.oleary@uwa.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019268
     1. Label: PRIDE project
     2. Name: Rubisco acetylation stoichiometry in Arabidopsis Thaliana