PXD019265

PXD019265 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of the response of Pseudomonas aeruginosa to gallium maltolate exposure
Description	The semi-metallic element gallium was initially introduced therapeutically as a diagnostic tool for the detection of cancerous tissue and was subsequently incorporated into a number of gallium-based anticancer agents. Gallium-based drugs have since been repurposed as antibacterial therapies and have shown significant potential as an alternative treatment option against resistant pathogens. The activity of gallium (Ga3+) is a result of its chemical similarity to ferric iron (Fe3+) and substitution into iron-dependent pathways. Gallium is incapable of reducing to the 2+ form in typical physiological environments and therefore inhibits iron metabolism vital for bacterial growth. Gallium maltolate (GaM) is an enhanced, novel formulation of gallium, consisting of a central gallium cation coordinated to three maltolate ligands, [Ga(Maltol-1H)3]. This study implemented a label-free quantitative proteomic approach to observe the effect of GaM on the opportunistic pathogen Pseudomonas aeruginosa. Analysis of the changes of the P. aeruginosa proteome in response to GaM exposure revealed how inadequate supplies of iron within the cell may explain the increase in abundance of proteins involved in iron acquisition and storage. A key finding of this study however, was the decreased abundance of proteins associated with quorum-sensing and swarming motility. These processes are a fundamental component of bacterial virulence and dissemination and hence explain a potential role of GaM in the treatment of P. aeruginosa infection.
HostingRepository	PRIDE
AnnounceDate	2022-06-27
AnnouncementXML	Submission_2022-06-27_06:36:05.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magdalena Piatek
SpeciesList	scientific name: Galleria mellonella; NCBI TaxID: 7137;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 16:52:08	ID requested
⏵ 1	2022-06-27 06:36:05	announced

Dataset with its publication pending

submitter keyword: Antimicrobial

Bacterial

Galleria

Gallium

Pseudomonas

Proteomics

Profesor Kevin Kavanagh
contact affiliation	Medical Mycology Lab Biology Department Maynooth University Ireland
contact email	Kevin.Kavanagh@mu.ie
lab head
Magdalena Piatek
contact affiliation	Maynooth University
contact email	magdalena.piatek@mu.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD019265

PRIDE project URI

• PRIDE
  1. PXD019265
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of the response of Pseudomonas aeruginosa to gallium maltolate exposure