PXD019258

PXD019258 is an original dataset announced via ProteomeXchange.

Title	The impact of biomaterial cell contact on the immunopeptidome
Description	Biomaterials play an increasing role in clinical applications and regenerative medicine. Modern biomaterials should imitate the function of damaged tissue without triggering an immune response, initiate self-regeneration of the body and gradually degrade after implantation. The immune system is now well recognized to play a major role in influencing the biocompatibility of implanted medical devices. To develop a better understanding of the effects of biomaterials on the immune response, we have developed a highly sensitive novel test system capable of examining changes in the immune system by biomaterial. Here, we evaluate for the first time the immunopeptidome, a highly sensitive system that reflects cancer transformation, virus or drug influences and passes these changes directly to T cells, as a test system to examine the effects of contact with materials. Since monocytes are one of the first immune cells reacting to biomaterials, we have tested the influence of different materials - stainless steel, aluminum, zinc, high-density polyethylene, polyurethane films containing zinc diethyldithiocarbamate, copper, and zinc sulphate - on the monocytic THP-1 cell line. Material-associated peptides were identified after stimulation with all material types examined. The magnitude of induced changes in the immunopeptidome after the stimulation appears comparable to that of bacterial lipopolysaccharides (LPS). The source proteins of many detected peptides are associated with cytotoxicity, fibrosis, autoimmunity, inflammation and cellular stress. Considering all tested materials, it was found that the LPS-induced cytotoxicity-, inflammation- and cellular stress-associated HLA class I peptides were mainly induced by aluminum and HLA class II peptides mainly by stainless steel. These findings provide some of the first insights into the effects on the immunopeptidome by biomaterials. A more thorough understanding of these effects may enable the design of more biocompatible implant materials using in vitro models in future. This may be achieved through developing a deeper understanding of possible immune responses induced by biomaterials such as fibrosis, inflammation, cytotoxicity, and autoimmune reactions.
HostingRepository	PRIDE
AnnounceDate	2020-12-01
AnnouncementXML	Submission_2021-01-06_06:28:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Ghosh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 04:46:42	ID requested
1	2020-11-30 23:31:27	announced
⏵ 2	2021-01-06 06:28:08	announced	2021-01-06: Updated publication reference for PubMed record(s): 33392160.

Ghosh M, Hartmann H, Jakobi M, M, ä, rz L, Bichmann L, Freudenmann LK, M, ü, hlenbruch L, Segan S, Rammensee HG, Schneiderhan-Marra N, Shipp C, Stevanovi, ć S, Joos TO, The Impact of Biomaterial Cell Contact on the Immunopeptidome. Front Bioeng Biotechnol, 8():571294(2020) [pubmed]

submitter keyword: Biomaterials, Biocompatibility, Biocompatibility assay, Monocytes, Foreign body response, immunopeptidomics, mass spectrometry, Host response

Stefan Stevanović
contact affiliation	c. University of Tübingen, Department of Immunology, Institute of Cell Biology, Auf der Morgenstelle 15, Tübingen 72076, Germany
contact email	stefan.stevanovic@uni-tuebingen.de
lab head
Michael Ghosh
contact affiliation	Department of Immunology, Tübingen
contact email	Michaelghosh@gmx.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019258
     1. Label: PRIDE project
     2. Name: The impact of biomaterial cell contact on the immunopeptidome