PXD019257

PXD019257 is an original dataset announced via ProteomeXchange.

Title	Peptide profile of the storage proteins of chickpea, wheat, soybean analyzed with LC-MS/MS
Description	Aiming to reduce food spoilage, the present study developed novel highly active food-grade preservatives affecting a wide range of bacteria. For this purpose, storage proteins were extracted from food plants. After enzymatic hydrolysis by the digestive protease chymotrypsin, the peptide profiles were analyzed by ultrahigh-performance micro-liquid chromatography–triple quadrupole time-of-flight tandem mass spectrometry. Virtual screening identified 21 potential antimicrobial peptides in chickpea legumin. Among those, the peptides Leg1 (RIKTVTSFDLPALRFLKL) and Leg2 (RIKTVTSFDLPALRWLKL) exhibited antimicrobial activity against 16 different bacteria, including pathogens, spoilage-causing bacteria and two antibiotic-resistant strains. Minimum inhibitory concentrations (MIC) down to 15.6 µM indicated 10–1,000-fold higher activity of the novel antimicrobial peptides compared to conventional food preservatives. Moreover, Leg1 and Leg2 showed bactericidal activity in bacterial suspension and during the storage of raw pork meat.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:38.081.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie-Louise Heymich
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 04:12:24	ID requested
1	2021-01-10 23:32:20	announced
⏵ 2	2024-10-22 05:17:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: storage proteins,Chickpea, chymotryptic digestion, soybean, wheat, LC-MS/MS

Monika Pischetsrieder
contact affiliation	Chair of Food Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Germany
contact email	Monika.Pischetsrieder@fau.de
lab head
Marie-Louise Heymich
contact affiliation	Food Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nürnberg
contact email	marie-louise.heymich@fau.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD019257

PRIDE project URI

• PRIDE
  1. PXD019257
     1. Label: PRIDE project
     2. Name: Peptide profile of the storage proteins of chickpea, wheat, soybean analyzed with LC-MS/MS