PXD019251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry |
Description | Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 19 (CDK19), cyclin-C, and an N-terminal fragment of subunit 12 of the Mediator complex (MED12 1-100). Cross-linking was performed using disuccinimidyl suberate (DSS). These results were generated in the context of the study published as Klatt et al., A precisely positioned MED12 activation helix stimulates CDK8 kinase activity, Proc. Natl. Acad. Sci. USA 2020 (DOI: 10.1073/pnas.1917635117) with the associated data set PXD015394, but were not included in the article. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-18 |
AnnouncementXML | Submission_2020-05-18_04:45:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexander Leitner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-18 02:24:37 | ID requested | |
⏵ 1 | 2020-05-18 04:45:56 | announced | |
Publication List
Klatt F, Leitner A, Kim IV, Ho-Xuan H, Schneider EV, Langhammer F, Weinmann R, M, ü, ller MR, Huber R, Meister G, Kuhn CD, A precisely positioned MED12 activation helix stimulates CDK8 kinase activity. Proc Natl Acad Sci U S A, 117(6):2894-2905(2020) [pubmed] |
Keyword List
submitter keyword: Chemical cross-linking |
DSS |
structural proteomics |
mediator complex |
kinase |
Contact List
Claus-D. Kuhn |
contact affiliation | University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany |
contact email | Claus.Kuhn@uni-bayreuth.de |
lab head | |
Alexander Leitner |
contact affiliation | ETH Zurich, Institute of Molecular Systems Biology |
contact email | leitner@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD019251 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019251
- Label: PRIDE project
- Name: Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry