PXD019251

PXD019251 is an original dataset announced via ProteomeXchange.

Title	Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry
Description	Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 19 (CDK19), cyclin-C, and an N-terminal fragment of subunit 12 of the Mediator complex (MED12 1-100). Cross-linking was performed using disuccinimidyl suberate (DSS). These results were generated in the context of the study published as Klatt et al., A precisely positioned MED12 activation helix stimulates CDK8 kinase activity, Proc. Natl. Acad. Sci. USA 2020 (DOI: 10.1073/pnas.1917635117) with the associated data set PXD015394, but were not included in the article.
HostingRepository	PRIDE
AnnounceDate	2020-05-18
AnnouncementXML	Submission_2020-05-18_04:45:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Leitner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 02:24:37	ID requested
⏵ 1	2020-05-18 04:45:56	announced

Klatt F, Leitner A, Kim IV, Ho-Xuan H, Schneider EV, Langhammer F, Weinmann R, M, ü, ller MR, Huber R, Meister G, Kuhn CD, A precisely positioned MED12 activation helix stimulates CDK8 kinase activity. Proc Natl Acad Sci U S A, 117(6):2894-2905(2020) [pubmed]

submitter keyword: Chemical cross-linking

DSS

structural proteomics

mediator complex

kinase

Claus-D. Kuhn
contact affiliation	University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany
contact email	Claus.Kuhn@uni-bayreuth.de
lab head
Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD019251

PRIDE project URI

• PRIDE
  1. PXD019251
     1. Label: PRIDE project
     2. Name: Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry