PXD019249

PXD019249 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of mouse cerebellar granule cells after knockdown of YTHDF1
Description	To identify the target mRNAs of YTHDF1 in granule cells, we performed quantitative proteomic analysis. Mouse cerebellar granule cells were cultured and infected with lentiviral shYthdf1 or shCtrl. Proteins were extracted. After further processing, TMT-labeled peptides were subjected to LC-MS/MS analysis. We discovered 352 proteins that were differentially expressed altogether (change fold >1.2), among which 142 proteins were downregulated after YTHDF1 KD. This study provides a protein list which shows differentially expressed proteins after knockdown of YTHDF1 in granule cells.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:43:53.103.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Yu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-18 01:03:09	ID requested
⏵ 1	2022-02-15 11:43:54	announced

Yu J, She Y, Yang L, Zhuang M, Han P, Liu J, Lin X, Wang N, Chen M, Jiang C, Zhang Y, Yuan Y, Ji SJ, A Readers YTHDF1 and YTHDF2 Synergistically Control Cerebellar Parallel Fiber Growth by Regulating Local Translation of the Key Wnt5a Signaling Components in Axons. Adv Sci (Weinh), 8(22):e2101329(2021) [pubmed]

submitter keyword: YTHDF1

YTHDF2

Local translation

ShengJian Ji
contact affiliation	Department of Biology, Brain Research Center, Southern University of Science and Technology
contact email	jisj@SUSTech.edu.cn
lab head
Jun Yu
contact affiliation	Southern University of Science and Technology
contact email	11650001@mail.sustech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD019249

PRIDE project URI

• PRIDE
  1. PXD019249
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of mouse cerebellar granule cells after knockdown of YTHDF1