PXD019243 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Optimization of protocols for detection of de novo protein synthesis in whole blood samples via azide-alkyne cycloaddition |
Description | Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer to neuropsychiatry. Blood-based biomarkers indicative of changes in proteomes have long been held to be potentially useful with respect to disease prognosis and treatment. However, most effort has been focused on unlabelled plasma proteomics which includes non-myeloid origin proteins and no method of dynamically tagging acute changes in proteomes. Herein we report a method for evaluating de novo protein synthesis in whole blood liquid biopsies. Using a modification of the “bioorthogonal noncanonical amino acid tagging” (BONCAT) protocol, rodent whole blood samples were incubated with L-azidohomoalanine (AHA) to allow incorporation of this selectively reactive non-natural amino acid within nascent polypeptides. Overall, we present a rapid and convenient de novo protein synthesis assay usable with whole blood biopsies that can quantify translational change as well as identity of proteins differentially expressed that may be useful for clinical applications |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:13:08.087.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Janani Priya Venkatasubramani |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-17 18:14:58 | ID requested | |
1 | 2020-10-07 23:03:58 | announced | |
⏵ 2 | 2024-10-22 05:13:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Bowling HL, Kasper A, Patole C, Venkatasubramani JP, Leventer SP, Carmody E, Sharp K, Berry-Kravis E, Kirshenbaum K, Klann E, Bhattacharya A, Optimization of Protocols for Detection of De Novo Protein Synthesis in Whole Blood Samples via Azide-Alkyne Cycloaddition. J Proteome Res, 19(9):3856-3866(2020) [pubmed] |
10.1021/acs.jproteome.0c00299; |
Keyword List
submitter keyword: De novo protein synthesis, blood-based biomarkers, copper(I)-catalyzed click-chemistry, whole blood, ethylenediaminetetraacetic acid |
Contact List
Dr. Aditi Bhattacharya |
contact affiliation | Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India. |
contact email | aditi@instem.res.in |
lab head | |
Janani Priya Venkatasubramani |
contact affiliation | InStem |
contact email | jananipv209@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019243
- Label: PRIDE project
- Name: Optimization of protocols for detection of de novo protein synthesis in whole blood samples via azide-alkyne cycloaddition