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PXD019243

PXD019243 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimization of protocols for detection of de novo protein synthesis in whole blood samples via azide-alkyne cycloaddition
DescriptionAberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer to neuropsychiatry. Blood-based biomarkers indicative of changes in proteomes have long been held to be potentially useful with respect to disease prognosis and treatment. However, most effort has been focused on unlabelled plasma proteomics which includes non-myeloid origin proteins and no method of dynamically tagging acute changes in proteomes. Herein we report a method for evaluating de novo protein synthesis in whole blood liquid biopsies. Using a modification of the “bioorthogonal noncanonical amino acid tagging” (BONCAT) protocol, rodent whole blood samples were incubated with L-azidohomoalanine (AHA) to allow incorporation of this selectively reactive non-natural amino acid within nascent polypeptides. Overall, we present a rapid and convenient de novo protein synthesis assay usable with whole blood biopsies that can quantify translational change as well as identity of proteins differentially expressed that may be useful for clinical applications
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:08.087.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJanani Priya Venkatasubramani
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListbiotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-17 18:14:58ID requested
12020-10-07 23:03:58announced
22024-10-22 05:13:14announced2024-10-22: Updated project metadata.
Publication List
Bowling HL, Kasper A, Patole C, Venkatasubramani JP, Leventer SP, Carmody E, Sharp K, Berry-Kravis E, Kirshenbaum K, Klann E, Bhattacharya A, Optimization of Protocols for Detection of De Novo Protein Synthesis in Whole Blood Samples via Azide-Alkyne Cycloaddition. J Proteome Res, 19(9):3856-3866(2020) [pubmed]
10.1021/acs.jproteome.0c00299;
Keyword List
submitter keyword: De novo protein synthesis, blood-based biomarkers, copper(I)-catalyzed click-chemistry, whole blood, ethylenediaminetetraacetic acid
Contact List
Dr. Aditi Bhattacharya
contact affiliationInstitute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India.
contact emailaditi@instem.res.in
lab head
Janani Priya Venkatasubramani
contact affiliationInStem
contact emailjananipv209@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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