PXD019233

PXD019233 is an original dataset announced via ProteomeXchange.

Title	chronic exposure to tramadol induces oxidative damage, inflammation based on mice serum proteome
Description	Peptides were labeled with TMT reagents based on the manufacturer’s instructions (Thermo Fisher Scientifific). To quantify six samples, each aliquot (100 µg of peptide equivalent) was reacted with one tube of TMT reagent. After the sample was dissolved in 100 µL of 0.05 M tetraethylammonium bromide (TEAB) solution, pH 8.5, the TMT reagent was dissolved in 41 µL of anhydrous acetonitrile. The mixture was incubated at room temperature for 1 hour.Then, 8 µL of 5% hydroxylamine was added to the sample and incubated for 15 min to quench the reaction. The 6-plex labeled samples were pooled together and lyophilized. The TMT-labeled peptides mixture was fractionated using a Waters XBridge BEH130 column (C18, 3.5 µm, 2.1 × 150 mm) on an Agilent 1290 high-performance liquid chromatography(HPLC) operating at 0.3 mL/min.
HostingRepository	iProX
AnnounceDate	2020-05-16
AnnouncementXML	Submission_2023-03-01_18:25:55.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lina Gao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-16 04:55:50	ID requested
1	2020-05-16 04:57:23	announced
⏵ 2	2023-03-01 18:25:57	announced	2023-03-02: Update publication information.

Jiang S, Liu G, Yuan H, Xu E, Xia W, Zhang X, Liu J, Gao L, Changes on proteomic and metabolomic profile in serum of mice induced by chronic exposure to tramadol. Sci Rep, 11(1):1454(2021) [pubmed]

Xia W, Cao Z, Zhang X, Gao L, A Proteomics Study on the Mechanism of Nutmeg-Induced Hepatotoxicity. Molecules, 26(6):(2021) [pubmed]

submitter keyword: mice, serum, proteome

Lina Gao
contact affiliation	China Medical University
contact email	gaolina0114@sina.com
lab head
Lina Gao
contact affiliation	China Medical University
contact email	gaolina0114@sina.com
dataset submitter

iProX dataset URI