PXD019215

PXD019215 is an original dataset announced via ProteomeXchange.

Title	MC4 crosslinking of rhodopsin and GRK1 complex
Description	G protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate agonist bound GPCRs, thereby switching the signal from G protein-dependent to arrestin-dependent pathways, including receptor internalization and downregulation. There are currently no high-resolution details known about how a GRK recognizes an activated receptor, but most functional studies indicate that its unique N-terminus is key to agonist-dependent phosphorylation. Herein, we report the 7.2 Å cryo-electron microscopy (EM) single particle reconstruction of the rhodopsin-rhodopsin kinase (GRK1) complex. The structure reveals a 1:1 assembly with multiple contact points between the GRK1 kinase domain and rhodopsin, the most prominent being the insertion of the GRK1 N-terminal helix directly into the cytoplasmic cleft formed by rhodopsin in its active state. Cross-linking coupled with mass spectrometry, along with functional studies, confirmed the observed interface between the proteins and revealed regions of the complex that are dynamic.
HostingRepository	PRIDE
AnnounceDate	2021-07-15
AnnouncementXML	Submission_2021-07-26_07:03:35.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manolo Plasencia
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-15 00:14:40	ID requested
1	2021-07-14 21:59:55	announced
⏵ 2	2021-07-26 07:03:36	announced	2021-07-26: Updated publication reference for PubMed record(s): 34262173.

Chen Q, Plasencia M, Li Z, Mukherjee S, Patra D, Chen CL, Klose T, Yao XQ, Kossiakoff AA, Chang L, Andrews PC, Tesmer JJG, Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature, 595(7868):600-605(2021) [pubmed]

submitter keyword: Crosslinking, MC4, GPCR, rhodopsin, GRK1, rhodopsin kinase

Philip C Andrews
contact affiliation	University of Michigan Medical School, Department of Biological Chemistry, Ann Arbor, MI
contact email	andrewsp@umich.edu
lab head
Manolo Plasencia
contact affiliation	University of Michigan Medical School
contact email	plasenci@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD019215

PRIDE project URI

• PRIDE
  1. PXD019215
     1. Label: PRIDE project
     2. Name: MC4 crosslinking of rhodopsin and GRK1 complex