PXD019208 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein S-Nitrosylation of host and viral proteins upon fibroblast infection with Human Cytomegalovirus |
Description | Human Cytomegalovirus (HCMV) is a ubiquitous pathogen that has co-evolved with its host and in doing so, is highly efficient in undermining antiviral responses that limit successful infections. As a result, HCMV infections are highly problematic in individuals with weakened or underdeveloped immune systems including transplant recipients and newborns. Understanding how HCMV controls the microenvironment of an infected cell so as to favor productive replication is of critical importance. To this end, we took an unbiased proteomics approach to identify the highly reversible, stress induced, post-translational modification (PTM), protein S-nitrosylation, on viral proteins to determine the biological impact on viral replication. We identified protein S-nitrosylation of 13 viral proteins during infection of highly permissive fibroblasts. One of these proteins, pp71, is critical for efficient viral replication, as it undermines host antiviral responses, including STING activation. By exploiting site-directed mutagenesis of the specific amino acids we identified in pp71 as protein S-nitrosylated, we found this pp71 PTM diminishes its ability to undermine antiviral responses induced by the STING pathway. Our results suggest a model in which protein S-nitrosylation may function as a host response to viral infection that limits viral spread. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:08:20.133.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ebbing de Jong |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-14 18:57:38 | ID requested | |
1 | 2020-07-02 23:26:11 | announced | |
2 | 2020-09-28 00:30:39 | announced | 2020-09-28: Updated publication reference for PubMed record(s): 32581105. |
⏵ 3 | 2024-10-22 05:08:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1128/jvi.00033-20; |
Nukui M, Roche KL, Jia J, Fox PL, Murphy EA, Protein S-Nitrosylation of Human Cytomegalovirus pp71 Inhibits Its Ability To Limit STING Antiviral Responses. J Virol, 94(17):(2020) [pubmed] |
Keyword List
submitter keyword: HCMV, Protein S-Nitrosylation, cytomegalovirus |
Contact List
Eain Murphy |
contact affiliation | Associate Professor Microbiology and Immunology Department Upstate Medical University Weiskotten Hall Rm. 2273 766 Irving Avenue Syracuse, NY 13210 Tel 315.464.5870 |
contact email | murphye1@upstate.edu |
lab head | |
Ebbing de Jong |
contact affiliation | SUNY Upstate Medical University |
contact email | dejonge@upstate.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019208
- Label: PRIDE project
- Name: Protein S-Nitrosylation of host and viral proteins upon fibroblast infection with Human Cytomegalovirus