⮝ Full datasets listing

PXD019202

PXD019202 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism
DescriptionHere, we present the crystal structure of the catalytic domain of M5 bound to two magnesium ions, which reveals the role of each catalytic site acidic residue in metal binding. We further use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to investigate the possibility of structural rearrangements that would allow cleavage of the 5’-strand, and use small-angle X-ray scattering (SAXS) to study the M5 binding to the pre-5S rRNA substrate. This study provides new details on the M5 mechanism used for cleavage of the dsRNA substrate, which proceeds via two metal ions and structural rearrangements of both M5 and the pre-5S rRNA substrate.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:05.732.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaxime BOURGUET
SpeciesList scientific name: Geobacillus stearothermophilus; NCBI TaxID: 1422;
ModificationListmonohydroxylated residue
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-14 06:01:47ID requested
12021-02-10 09:15:20announced
22023-11-14 08:51:06announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RNAse, native-MS, HDX-MS
Contact List
Sarah CIANFERANI
contact affiliationLSMBO - IPHC - CNRS
contact emailsarah.cianferani@unistra.fr
lab head
Maxime BOURGUET
contact affiliationLSMBO - IPHC / CNRS
contact emailmaxime.bourguet@etu.unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD019202
PRIDE project URI
Repository Record List
[ + ]