PXD019202 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism |
Description | Here, we present the crystal structure of the catalytic domain of M5 bound to two magnesium ions, which reveals the role of each catalytic site acidic residue in metal binding. We further use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to investigate the possibility of structural rearrangements that would allow cleavage of the 5’-strand, and use small-angle X-ray scattering (SAXS) to study the M5 binding to the pre-5S rRNA substrate. This study provides new details on the M5 mechanism used for cleavage of the dsRNA substrate, which proceeds via two metal ions and structural rearrangements of both M5 and the pre-5S rRNA substrate. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:05.732.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maxime BOURGUET |
SpeciesList | scientific name: Geobacillus stearothermophilus; NCBI TaxID: 1422; |
ModificationList | monohydroxylated residue |
Instrument | SYNAPT G2-Si |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-14 06:01:47 | ID requested | |
1 | 2021-02-10 09:15:20 | announced | |
⏵ 2 | 2023-11-14 08:51:06 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNAse, native-MS, HDX-MS |
Contact List
Sarah CIANFERANI |
contact affiliation | LSMBO - IPHC - CNRS |
contact email | sarah.cianferani@unistra.fr |
lab head | |
Maxime BOURGUET |
contact affiliation | LSMBO - IPHC / CNRS |
contact email | maxime.bourguet@etu.unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019202
- Label: PRIDE project
- Name: Bacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism