PXD019202

PXD019202 is an original dataset announced via ProteomeXchange.

Title	Bacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism
Description	Here, we present the crystal structure of the catalytic domain of M5 bound to two magnesium ions, which reveals the role of each catalytic site acidic residue in metal binding. We further use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to investigate the possibility of structural rearrangements that would allow cleavage of the 5’-strand, and use small-angle X-ray scattering (SAXS) to study the M5 binding to the pre-5S rRNA substrate. This study provides new details on the M5 mechanism used for cleavage of the dsRNA substrate, which proceeds via two metal ions and structural rearrangements of both M5 and the pre-5S rRNA substrate.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:05.732.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maxime BOURGUET
SpeciesList	scientific name: Geobacillus stearothermophilus; NCBI TaxID: 1422;
ModificationList	monohydroxylated residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-14 06:01:47	ID requested
1	2021-02-10 09:15:20	announced
⏵ 2	2023-11-14 08:51:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: RNAse, native-MS, HDX-MS

Sarah CIANFERANI
contact affiliation	LSMBO - IPHC - CNRS
contact email	sarah.cianferani@unistra.fr
lab head
Maxime BOURGUET
contact affiliation	LSMBO - IPHC / CNRS
contact email	maxime.bourguet@etu.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019202
     1. Label: PRIDE project
     2. Name: Bacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism