PXD019170

PXD019170 is an original dataset announced via ProteomeXchange.

Title	Subcellular localization of the J-protein Sis1 regulates the heat shock response
Description	How heat shock inducesthe heat shock response (HSR) – a gene expression program encoding chaperones and other protein homeostasis (proteostasis) factors –remains an unresolved question in eukaryotic cell biology. Here we show that subcellular localization of the conserved J-protein Sis1 is a key regulator of the HSRin yeast. Under nonstress conditions, nucleoplasmic Sis1 promotes interaction between the chaperone Hsp70 and the transcription factor Hsf1to repress the HSR. Heat shock triggers Sis1 to localize to the nucleolar periphery and condense on the ER surface with the ribosome quality control complex. Sis1 recruits the proteasome to this spatial network.Through localization dynamics, Sis1 relaysthe condition of the proteome to Hsf1.Thus, the activation state of the HSR is built into spatial organization of the proteostasis network.
HostingRepository	PRIDE
AnnounceDate	2021-03-24
AnnouncementXML	Submission_2021-03-24_12:37:23.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-14 03:28:21	ID requested
⏵ 1	2021-03-24 12:37:24	announced

Feder ZA, Ali A, Singh A, Krakowiak J, Zheng X, Bindokas VP, Wolfgeher D, Kron SJ, Pincus D, Subcellular localization of the J-protein Sis1 regulates the heat shock response. J Cell Biol, 220(1):(2021) [pubmed]

submitter keyword: Q-Exactive, J-protein, SIS1, , HSF1, heat shock, Saccharomyces cerevisiae

David Pincus
contact affiliation	Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
contact email	pincus@uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019170
     1. Label: PRIDE project
     2. Name: Subcellular localization of the J-protein Sis1 regulates the heat shock response