PXD019152 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DDI2 is a ubiquitin-directed endoprotease, responsible for cleavage of transcription factor NRF1 |
Description | The Ddi1/DDI2 proteins are ubiquitin shuttling factors, implicated in a variety of cellular functions. In addition to ubiquitin-binding and ubiquitin-like domains, they contain a conserved region with similarity to retroviral proteases, but whether and how DDI2 functions as a protease has remained unknown. Here we show that DDI2 knock-out cells are sensitive to proteasome inhibition and accumulate high-molecular weight, ubiquitylated proteins that are poorly degraded by the proteasome. These proteins are targets for the protease activity of purified DDI2. No evidence for DDI2 acting as a de-ubiquitylating enzyme was uncovered, which could suggest that it cleaves the ubiquitylated protein itself. In support of this idea, cleavage of transcription factor NRF1 is known to require DDI2 activity in vivo. We show that DDI2 is indeed capable of cleaving NRF1 in vitro, but only when NRF1 protein is poly-ubiquitylated. Together, these data suggest that DDI2 is a ubiquitin-directed endo-protease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:07:47.170.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michele Puglia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-12 22:38:02 | ID requested | |
1 | 2020-06-23 23:46:46 | announced | |
⏵ 2 | 2024-10-22 05:07:48 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.molcel.2020.05.035; |
Dirac-Svejstrup AB, Walker J, Faull P, Encheva V, Akimov V, Puglia M, Perkins D, K, ΓΌ, mper S, Hunjan SS, Blagoev B, Snijders AP, Powell DJ, Svejstrup JQ, DDI2 Is a Ubiquitin-Directed Endoprotease Responsible for Cleavage of Transcription Factor NRF1. Mol Cell, 79(2):332-341.e7(2020) [pubmed] |
Keyword List
submitter keyword: UbiSite, PRM,DDI2, Ubiquitin, HpH, LC-MS/MS |
Contact List
Blagoy Blagoev |
contact affiliation | University of Southern Denmark (SDU), Department of Biochemistry and Molecular Biology (BMB) |
contact email | bab@bmb.sdu.dk |
lab head | |
Michele Puglia |
contact affiliation | University of Southern Denmark |
contact email | michelep@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019152
- Label: PRIDE project
- Name: DDI2 is a ubiquitin-directed endoprotease, responsible for cleavage of transcription factor NRF1