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PXD019140

PXD019140 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparison between the proteome of Escherichia coli single colony and during liquid culture
DescriptionMost of the proteomic studies done so far uses bacterial cells harvested from liquid culture media. However it is widely accepted that many important determinants associated with virulence and host cell adhesion are exclusively expressed during growth on solid media. As an attempt to complement previous studies, in this study we compare the proteome coverage of Escherichia coli K12 from a single colony on solid media with those at different growth phases in liquid culture; i.e. early log, mid log, early - and late stationary growth phases. A total of 2044 protein groups covering approximately 47% of the total proteome were identified across all studied conditions; 1650 number of proteins identified from single colonies and 1679 proteins from liquid cultured cells. Label-free quantitative analysis revealed that single colony proteome in a solid agar differs largely from that in liquid culture. The presence of the Suf-operon, involved in iron mobilisation and swarming motility were associated exclusively with single colony profiles. Whereas proteins involved in motility such as MotA, MotB, fliH, flip, fliD and fliJ were associated exclusively to cells grown in liquid culture. The data presented here provide a valuable resource for understanding the role of key proteins within microenvironments surrounding E.coli single colonies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:27:48.662.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterjohn Iradukunda
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-12 19:03:16ID requested
12021-09-08 23:32:20announced
22024-10-22 05:27:50announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.mex.2021.101277;
Fortuin S, Iradukunda J, Nel AJ, Blackburn JM, Soares NC, single colony. MethodsX, 8():101277(2021) [pubmed]
Keyword List
submitter keyword: protein extraction, bacterial proteome, Ecoli, Mass spectrometry,Single-colony, Proteomics
Contact List
Jonathan Blackburn
contact affiliationDepartment:Intergrated biomedical sciences,Division:Chemical biology,Institute of infectious diseases and Molecular medicine(IDM),Faculty of health science, University of Cape Town, South Africa
contact emailJonathan.blackburn@uct.ac.za
lab head
john Iradukunda
contact affiliationUniversity of Cape Town
contact emailirdjoh001@myuct.ac.za
dataset submitter
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Dataset FTP location
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