PXD019136 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A bioenergetic shift is required for spermatogonial differentiation |
Description | A bioenergetic balance between glycolysis and mitochondrial respiration is particularly important for stem cell fate specification. It however remains to be determined whether undifferentiated spermatogonia switch their preference of bioenergy production during differentiation. In this study, we found that ATP generation in spermatogonia was gradually increased upon retinoic acid-induced differentiation. To accommodate this elevated energy demand, retinoic acid signaling concomitantly switched ATP production in spermatogonia from glycolysis to mitochondrial respiration, accompanied by increased levels of reactive oxygen species. In addition, inhibition of glucose conversion to glucose-6-phosphate or pentose phosphate pathway blocked the formation of c-Kit+ differentiating germ cells, suggesting that metabolites produced from glycolysis are required for spermatogonial differentiation. We further demonstrated that the expression levels of several metabolic regulators and enzymes were significantly altered upon retinoic acid-induced differentiation by both RNA-seq analyses and quantitative proteomics. Taken together, our data unveil a critically regulated bioenergetic balance between glycolysis and mitochondrial respiration which is required for spermatogonial proliferation and differentiation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:27:12.212.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liangliang Sun |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-12 19:00:49 | ID requested | |
1 | 2021-09-08 18:10:55 | announced | |
⏵ 2 | 2024-10-22 05:27:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41421-020-0183-x; |
Chen W, Zhang Z, Chang C, Yang Z, Wang P, Fu H, Wei X, Chen E, Tan S, Huang W, Sun L, Ni T, Yang Y, Wang Y, A bioenergetic shift is required for spermatogonial differentiation. Cell Discov, 6():56(2020) [pubmed] |
Keyword List
submitter keyword: spermatogonial differentiation, OXPHOS,glycolysis |
Contact List
Liangliang Sun |
contact affiliation | Department of Chemistry, Michigan State University |
contact email | lsun@chemistry.msu.edu |
lab head | |
Liangliang Sun |
contact affiliation | Michigan State University |
contact email | lsun@chemistry.msu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019136
- Label: PRIDE project
- Name: A bioenergetic shift is required for spermatogonial differentiation