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PXD019136

PXD019136 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA bioenergetic shift is required for spermatogonial differentiation
DescriptionA bioenergetic balance between glycolysis and mitochondrial respiration is particularly important for stem cell fate specification. It however remains to be determined whether undifferentiated spermatogonia switch their preference of bioenergy production during differentiation. In this study, we found that ATP generation in spermatogonia was gradually increased upon retinoic acid-induced differentiation. To accommodate this elevated energy demand, retinoic acid signaling concomitantly switched ATP production in spermatogonia from glycolysis to mitochondrial respiration, accompanied by increased levels of reactive oxygen species. In addition, inhibition of glucose conversion to glucose-6-phosphate or pentose phosphate pathway blocked the formation of c-Kit+ differentiating germ cells, suggesting that metabolites produced from glycolysis are required for spermatogonial differentiation. We further demonstrated that the expression levels of several metabolic regulators and enzymes were significantly altered upon retinoic acid-induced differentiation by both RNA-seq analyses and quantitative proteomics. Taken together, our data unveil a critically regulated bioenergetic balance between glycolysis and mitochondrial respiration which is required for spermatogonial proliferation and differentiation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:27:12.212.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiangliang Sun
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-12 19:00:49ID requested
12021-09-08 18:10:55announced
22024-10-22 05:27:13announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41421-020-0183-x;
Chen W, Zhang Z, Chang C, Yang Z, Wang P, Fu H, Wei X, Chen E, Tan S, Huang W, Sun L, Ni T, Yang Y, Wang Y, A bioenergetic shift is required for spermatogonial differentiation. Cell Discov, 6():56(2020) [pubmed]
Keyword List
submitter keyword: spermatogonial differentiation, OXPHOS,glycolysis
Contact List
Liangliang Sun
contact affiliationDepartment of Chemistry, Michigan State University
contact emaillsun@chemistry.msu.edu
lab head
Liangliang Sun
contact affiliationMichigan State University
contact emaillsun@chemistry.msu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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