PXD019134

PXD019134 is an original dataset announced via ProteomeXchange.

Title	Revealing the venomous secrets of the spider’s web
Description	Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomics and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web; and the presence of fatty acids in the web may be responsible mechanism for open the way to the web-toxins for accessing the interior of prey's body, as showed here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups - Araneomorphae and Mygalomorphae; and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems; these data also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins.
HostingRepository	PRIDE
AnnounceDate	2020-07-06
AnnouncementXML	Submission_2020-07-05_22:16:39.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019134
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	José Roberto Aparecido dos Santos-Pinto
SpeciesList	scientific name: Trichonephila clavipes; NCBI TaxID: 2585209;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 18:59:53	ID requested
⏵ 1	2020-07-05 22:16:40	announced

Esteves FG, Dos Santos-Pinto JRA, Ferro M, Sialana FJ, Smidak R, Rares LC, Nussbaumer T, Rattei T, Bilban M, Bacci J, ú, nior M, Lubec G, Palma MS, Revealing the Venomous Secrets of the Spider's Web. J Proteome Res, 19(8):3044-3059(2020) [pubmed]

submitter keyword: spider silk-producing glands, web silk toxins, de novo transcriptome assembly, LCMS-based proteomics, neurotoxins

José Roberto Aparecido dos Santos-Pinto
contact affiliation	Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP, 13500 Brazil.
contact email	jrbio08@gmail.com
lab head
José Roberto Aparecido dos Santos-Pinto
contact affiliation	São Paulo State University - UNESP
contact email	jrbio08@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019134

PRIDE project URI

• PRIDE
  1. PXD019134
     1. Label: PRIDE project
     2. Name: Revealing the venomous secrets of the spider’s web