PXD019132

PXD019132 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome
Description	Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, using whole cell and membrane proteomics, we show that the deletion of carotenoid production in Pantoea sp. YR343 results in altered membrane protein distribution and abundance. Moreover, we observe significant differences in the protein composition of detergent-resistant membrane fractions from wildtype and mutant cells, consistent
HostingRepository	MassIVE
AnnounceDate	2020-09-22
AnnouncementXML	Submission_2020-09-22_07:05:25.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Abraham
SpeciesList	scientific name: Pantoea sp. YR343; NCBI TaxID: 1144341;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 12:29:30	ID requested
⏵ 1	2020-09-22 07:05:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: membrane proteome, plant-associated microbe

submitter keyword: membrane proteome, plant-associated microbe

Contact List

Jennifer Morrell-Falvey
contact affiliation	ORNL
contact email	morrelljl1@ornl.gov
lab head
Paul Abraham
contact affiliation	ORNL
contact email	pav@ornl.gov
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085402/