PXD019126

PXD019126 is an original dataset announced via ProteomeXchange.

Title	Multiple myeloma microenvironment proteomics LC-MS/MS
Description	Although the "seed and soil" hypothesis was proposed by Stephen Paget at the end of the 19th century, where he said that tumor cells (seeds) need a propitious medium (soil) to be able to establish metastases, only recently the tumor microenvironment started to be more studied in the field of Oncology. Multiple myeloma (MM), a malignancy of plasma cells, can be considered one of the types of cancers where there is more evidence in the literature of the central role that the bone marrow (BM) microenvironment plays, contributing to proliferation, survival, migration and drug resistance of tumor cells. Despite all advances in the therapeutic arsenal for MM treatment in the last years, the disease remains incurable. Thus, studies aiming a better understanding of the pathophysiology of the disease, as well as searching for new therapeutic targets are necessary and welcome. Therefore, the present study aimed to evaluate the protein expression profiling of mononuclear cells derived from BM of MM patients in comparison with these same cell types derived from healthy individuals, in order to fill this gap in MM treatment. Proteomic analysis was performed using the mass spectrometry technique and further analyses were done using bioinformatics tools, to identify dysregulated biological pathways and/or processes in the BM microenvironment of patients with MM as a result of the disease. Among the pathways identified in this study, we can highlight an upregulation of proteins related to protein biosynthesis, especially chaperone proteins, in patients with MM. Additionally, we also found an upregulation of several proteins involved in energy metabolism, which is one of the cancer hallmarks. Finally, with regard to the downregulated proteins, we can highlight mainly those involved in different pathways of the immune response, corroborating the data that has demonstrated that the immune system of MM is impaired and, therefore, the immunotherapies that have been studied recently for the treatment of the disease are extremely necessary in the search for a control and a cure for these patients who live with the disease.
HostingRepository	PRIDE
AnnounceDate	2021-04-15
AnnouncementXML	Submission_2021-04-14_21:15:31.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rodrigo Carlini Fernando
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 05:59:31	ID requested
1	2021-04-14 07:02:18	announced
⏵ 2	2021-04-14 21:15:32	announced	2021-04-15: Updated project metadata.

10.3389/FONC.2021.563384;

Fernando RC, de Carvalho F, Leme AFP, Colleoni GWB, Tumor Microenvironment Proteomics: Lessons From Multiple Myeloma. Front Oncol, 11():563384(2021) [pubmed]

submitter keyword: proteomics,Multiple myeloma, proteome, tumor microenvironment

Gisele Wally Braga Colleoni
contact affiliation	Full professor, Federal University of São Paulo, Brazil.
contact email	gcolleoni@unifesp.br
lab head
Rodrigo Carlini Fernando
contact affiliation	Federal University of São Paulo
contact email	rodrigocfernando@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD019126

PRIDE project URI

• PRIDE
  1. PXD019126
     1. Label: PRIDE project
     2. Name: Multiple myeloma microenvironment proteomics LC-MS/MS