PXD019117

PXD019117 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic profiling of hippocampus from offspring rats exposed to prenatal stress
Description	Prenatal stress (PS) could cause depression of offprsing. Here, relative quantitative phosphoproteomics of hippocampus of PS-S and CON offspring rats were performed using LC-MS/MS to confirm known pathways and identify new areas of depression. A total of 6,790 phosphopeptides, 9,817 phosphorylation sites and 2978 phosphoproteins were detected. Among the 2978 phosphoproteins, 1760 (59.09%) with more than two phosphorylated sites, ENSRNOP00000023460 protein has more than 117 phosphorylated sites, and the average distribution of modification sites per 100 amino acids was 2.97. There were 197 different phophpeptides, including 140 increased phosphopeptides and 57 decreased phosphopeptides in PS-S offspring rats when compared to the CON offspring rats. These differential phosphopeptides corresponded to 100 upregulated and 44 downregulated phosphoproteins respectively. GO enrichment analysis these different phosphoproteins in the first five enrichments of CC category, MF category and BP category were involved in a total of 35 different phosphoproteins and these phosphoproteins were mainly related to myelin, microtubule and synapse associated proteins. The first five enrichment of KEGG pathways were found to be involved in many essential biological pathways, and the first five pathways included amphetamine addiction, insulin secretion, cushing syndrome and circadian entrainment signaling pathway. And these first five pathways were related to nine proteins including Adcy9, Apc, Cacna1c, Camk2a, Camk2b, Camk2g, Ctnnd2, Grin2a, Stx1a. Further research is needed to understand the precise role of these pathways in the depressive-like behavior of offspring rats caused by PS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:47.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongli Sun
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 01:37:59	ID requested
1	2021-12-30 14:36:04	announced
⏵ 2	2024-10-22 05:30:49	announced	2024-10-22: Updated project metadata.

Li Q, Cai D, Huang H, Zhang H, Bai R, Zhao X, Sun H, Qin P, Phosphoproteomic profiling of the hippocampus of offspring rats exposed to prenatal stress. Brain Behav, 11(10):e2233(2021) [pubmed]

10.1002/brb3.2233;

submitter keyword: hippocampus,Rat, LC-MS/MS

Hongli Sun
contact affiliation	Xi’an Children’s Hospital (The Affiliated Children’s Hospital of Xi’an Jiaotong University)
contact email	sunhongligo@yeah.net
lab head
Hongli Sun
contact affiliation	Xi'an Children's Hospital
contact email	sunhongligo@yeah.net
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019117
     1. Label: PRIDE project
     2. Name: Phosphoproteomic profiling of hippocampus from offspring rats exposed to prenatal stress