PXD019114

PXD019114 is an original dataset announced via ProteomeXchange.

Title	Tandem mass tags (TMT)–based quantitative proteomics approach quantified in 2-hydroxyisobutyrylation site of rice spikelets and Ustilaginoidea virens by mass spectrometry.
Description	Rice false smut, caused by the pathogenic ascomycete fungus Ustilaginoidea virens (teleomorph: Villosiclava virens), is one of the most devastating grain diseases in the majority of rice-growing areas of the world. Lysine 2-hydroxyisobutyrylation (Khib) is a novel post-translational modification (PTM), which plays an important role in active gene transcription and cellular proliferation in eukaryotes. However, its function remains unknown in phytopathogens and plant. Here, we report a comprehensive identification of Khib in rice false smut fungus Ustilaginoidea virens and rice. By using a tandem mass tags (TMT)–based quantitative proteomics approach, 2-hydroxyisobutyrylation sites were identified in U. virens and rice.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:37.290.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyang Chen
SpeciesList	scientific name: Ustilaginoidea virens; NCBI TaxID: 1159556; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-12 01:37:43	ID requested
1	2023-06-06 01:10:32	announced
⏵ 2	2023-11-14 08:48:42	announced	2023-11-14: Updated project metadata.

Chen X, Li X, Li P, Chen X, Liu H, Huang J, Luo C, Hsiang T, Zheng L, Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence. J Integr Plant Biol, 63(2):409-425(2021) [pubmed]

submitter keyword: 2-hydroxyisobutyrylation

TMT

mass spectrometry

Lu zheng
contact affiliation	Huazhong Agricultural University
contact email	luzheng@mail.hzau.edu.cn
lab head
Xiaoyang Chen
contact affiliation	The Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
contact email	1149806052@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD019114

PRIDE project URI

• PRIDE
  1. PXD019114
     1. Label: PRIDE project
     2. Name: Tandem mass tags (TMT)–based quantitative proteomics approach quantified in 2-hydroxyisobutyrylation site of rice spikelets and Ustilaginoidea virens by mass spectrometry.