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PXD019095

PXD019095 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSmall RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria
DescriptionRapid modulation of gene expression is a key feature for the success of bacteria, particularly for those that rapidly have to adapt to different niches. The lifecycles of Photorhabdus and Xenorhabdus involve a mutualistic association with nematodes as well as an entomopathogenic phase1,2, both of which rely on the production of numerous specialized metabolites (SMs) 3,4. Several regulators have been previously implicated in the regulation of SM production in these genera3,4. However, the molecular underpinnings regulating SM production and the role of small regulatory RNAs (sRNAs) in this process are unknown. Here we describe the mechanism underlying RNA-mediated control of SM synthesis. We show that the Hfq-dependent sRNA, ArcZ, is an essential requirement for SM production. We discovered that ArcZ directly base-pairs with the mRNA encoding HexA, a key repressor of SM genes. We further demonstrate that the ArcZ regulon is not restricted to SM production, but rather modulates up to ~15% of the transcriptional output in both Photorhabdus and Xenorhabdus. Together, our study shows that sRNAs are crucial for SM production in these species, reveals previously unknown targets for biosynthetic pathway manipulations, and offers a new tool for the (over)production, isolation and identification of unknown natural products.
HostingRepositoryPRIDE
AnnounceDate2020-08-25
AnnouncementXMLSubmission_2020-08-25_06:20:49.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD019095
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTimo Glatter
SpeciesList scientific name: Photorhabdus laumondii; NCBI TaxID: 2218628;
ModificationListDeamidated; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-11 10:19:18ID requested
12020-08-25 06:20:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Photorhabdus, ArcZ, Hfq, HexA, sRNA
Contact List
Helge Bode
contact affiliationMolekulare Biotechnologie Fachbereich Biowissenschaften Buchmann Insitutee for Molecular Life Sciences Goethe Universität Frankfurt
contact emailh.bode@bio.uni-frankfurt.de
lab head
Timo Glatter
contact affiliationMPI Marburg
contact emailglatter@mpi-marburg.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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