PXD019095

PXD019095 is an original dataset announced via ProteomeXchange.

Title	Small RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria
Description	Rapid modulation of gene expression is a key feature for the success of bacteria, particularly for those that rapidly have to adapt to different niches. The lifecycles of Photorhabdus and Xenorhabdus involve a mutualistic association with nematodes as well as an entomopathogenic phase1,2, both of which rely on the production of numerous specialized metabolites (SMs) 3,4. Several regulators have been previously implicated in the regulation of SM production in these genera3,4. However, the molecular underpinnings regulating SM production and the role of small regulatory RNAs (sRNAs) in this process are unknown. Here we describe the mechanism underlying RNA-mediated control of SM synthesis. We show that the Hfq-dependent sRNA, ArcZ, is an essential requirement for SM production. We discovered that ArcZ directly base-pairs with the mRNA encoding HexA, a key repressor of SM genes. We further demonstrate that the ArcZ regulon is not restricted to SM production, but rather modulates up to ~15% of the transcriptional output in both Photorhabdus and Xenorhabdus. Together, our study shows that sRNAs are crucial for SM production in these species, reveals previously unknown targets for biosynthetic pathway manipulations, and offers a new tool for the (over)production, isolation and identification of unknown natural products.
HostingRepository	PRIDE
AnnounceDate	2020-08-25
AnnouncementXML	Submission_2020-08-25_06:20:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019095
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Photorhabdus laumondii; NCBI TaxID: 2218628;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-11 10:19:18	ID requested
⏵ 1	2020-08-25 06:20:50	announced

Dataset with its publication pending

submitter keyword: Photorhabdus, ArcZ, Hfq, HexA, sRNA

Helge Bode
contact affiliation	Molekulare Biotechnologie Fachbereich Biowissenschaften Buchmann Insitutee for Molecular Life Sciences Goethe Universität Frankfurt
contact email	h.bode@bio.uni-frankfurt.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019095
     1. Label: PRIDE project
     2. Name: Small RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria