PXD019084

PXD019084 is an original dataset announced via ProteomeXchange.

Title	γ-glutamylcysteine (GGC) supplementation and p-LPS challenge causes distinct proteomic changes to primary airway epithelial cell models of Cystic Fibrosis
Description	Cystic fibrosis (CF) is a life limiting inherited condition associated with recurrent bacterial infections, inflammation, oxidative stress and loss of lung function. CF is characterised by deficiencies in both intra- and extracellular glutathione (GSH) levels. This GSH depletion is exacerbated by bacterial infections which further contributes to cellular oxidative stress and the inadequate control of inflammatory pathways in CF patients. A considerable body of research supports targeting the GSH biosynthesis pathway as a therapeutic strategy, however, current therapies have not demonstrated relevant improvements in CF clinical outcomes. It has previously shown that the immediate pre-cursor to glutathione, γ-glutamylcysteine (GGC) is effective in increasing intracellular levels of GSH in vivo in healthy humans. In this in-vitro primary cell line study, we study the proteomic changes involved in treating airway epithelial cells with GGC both prior to and post-LPS challenge, and without LPS challenge.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:15.376.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Capraro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-11 07:51:09	ID requested
1	2021-09-09 01:25:49	announced
⏵ 2	2024-10-22 05:28:23	announced	2024-10-22: Updated project metadata.

Hewson CK, Capraro A, Wong SL, Pandzic E, Zhong L, Fernando BSM, Awatade NT, Hart-Smith G, Whan RM, Thomas SR, Jaffe A, Bridge WJ, Waters SA, -Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells. Antioxidants (Basel), 9(12):(2020) [pubmed]

10.3390/antiox9121204;

submitter keyword: LPS, glutathione, cystic fibrosis, airway epithelial cells,qLC-MS/MS, γ-glutamylcysteine, oxidative stress

Shafagh Waters
contact affiliation	Molecular and Integrative Cystic Fibrosis (miCF) Research Laboratory, School of Women’s and Children’s Health, UNSW Medicine, UNSW Sydney (lab head)
contact email	shafagh.waters@unsw.edu.au
lab head
Alexander Capraro
contact affiliation	UNSW Australia
contact email	a.capraro@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019084

PRIDE project URI

• PRIDE
  1. PXD019084
     1. Label: PRIDE project
     2. Name: γ-glutamylcysteine (GGC) supplementation and p-LPS challenge causes distinct proteomic changes to primary airway epithelial cell models of Cystic Fibrosis