PXD019083

PXD019083 is an original dataset announced via ProteomeXchange.

Title	A selective autophagy pathway for endocytic protein condensates
Description	Autophagy eliminates cytoplasmic content selected by autophagy receptors, which link cargoes to the membrane bound autophagosomal ubiquitin-like protein Atg8/LC3. Here, we discover a selective autophagy pathway for protein condensates formed by endocytic proteins. In this pathway, the endocytic yeast protein Ede1 functions as a selective autophagy receptor. Distinct domains within Ede1 bind Atg8 and mediate phase separation into condensates. Both properties are necessary for an Ede1-dependent autophagy pathway for endocytic proteins, which differs from regular endocytosis, does not involve other known selective autophagy receptors, but requires the core autophagy machinery. Cryo-electron tomography of Ede1-containing condensates – at the plasma membrane and in autophagic bodies – shows a phase-separated compartment at the beginning and end of the Ede1-mediated selective autophagy pathway. Our results imply an important role of autophagy in surveillance of membraneless compartments
HostingRepository	PRIDE
AnnounceDate	2020-11-23
AnnouncementXML	Submission_2020-11-23_07:05:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Wilfling
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	ubiquitinylated lysine; phosphorylated residue; sumoylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-11 07:50:51	ID requested
⏵ 1	2020-11-23 07:05:14	announced

Wilfling F, Lee CW, Erdmann PS, Zheng Y, Sherpa D, Jentsch S, Pfander B, Schulman BA, Baumeister W, A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits. Mol Cell, 80(5):764-778.e7(2020) [pubmed]

submitter keyword: selective autophagy,liquid-liquid phase separation, Ede1, Atg8

Florian Wilfling
contact affiliation	MPI of Biochemistry
contact email	fwilfling@biochem.mpg.de
lab head
Florian Wilfling
contact affiliation	Max Planck Institute of Biochemistry
contact email	fwilfling@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019083
     1. Label: PRIDE project
     2. Name: A selective autophagy pathway for endocytic protein condensates