PXD019079

PXD019079 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome fin(ger)prints of propylthiouracil interference with the thyroid system in zebrafish embryos as 3R alternative in environmental hazard assessment
Description	Endocrine disruption (ED) can trigger far-reaching effects on environmental populations, justifying a refusal of market approval for chemicals with ED properties. For the assessment of ED effects on the thyroid system, regulatory decisions mostly rely on amphibian studies. Here we present a rapid and reproducible data dependent proteomics approach for identifying comprehensive molecular signatures of interference with the thyroid system in zebrafish (Danio rerio) embryos as an alternative to animal testing. For this, we have analysed the thyroid peroxidase inhibitor 6-propyl-2-thiouracil (6-PTU) as model substances for anti-thyroidal activity in a modified zebrafish embryo toxicity test (zFET). These fingerprints allow for a definition of solid biomarkers as tools in screening approaches and for integration in chronic toxicity studies for suspect substances, such as the fish early life-stage test (OECD TG 210).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:53.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steve Ayobahan
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-11 07:11:38	ID requested
1	2021-01-15 00:16:01	announced
⏵ 2	2024-10-22 05:17:54	announced	2024-10-22: Updated project metadata.

Reinwald H, K, ö, nig A, Ayobahan SU, Alvincz J, Sipos L, G, ö, ckener B, B, ö, hle G, Shomroni O, Hollert H, Salinas G, Sch, ä, fers C, Eilebrecht E, Eilebrecht S, Toxicogenomic fin(ger)prints for thyroid disruption AOP refinement and biomarker identification in zebrafish embryos. Sci Total Environ, 760():143914(2021) [pubmed]

10.1016/j.scitotenv.2020.143914;

submitter keyword: thyroid hormone, Endocrine disruption,Zebrafish embryo, Ecotoxicological testing, LC-MS/MS

Dr. Sebastian Eilebrecht
contact affiliation	Fraunhofer Attract Eco’n’OMICs, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Auf dem Aberg 1, 57392 Schmallenberg.
contact email	sebastian.eilebrecht@ime.fraunhofer.de
lab head
Steve Ayobahan
contact affiliation	Fraunhofer IME
contact email	steve.ayobahan@ime.fraunhofer.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD019079

PRIDE project URI

• PRIDE
  1. PXD019079
     1. Label: PRIDE project
     2. Name: Quantitative proteome fin(ger)prints of propylthiouracil interference with the thyroid system in zebrafish embryos as 3R alternative in environmental hazard assessment