PXD019077

PXD019077 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of nucleoid-associated proteins isolated from Mycoplasma gallisepticum in different growth phases
Description	Mycoplasma gallisepticum (MG) is one of the smallest free-living and self-replicating organisms, it is characterized by lack of cell wall and reduced genome size. As a result of genome reduction, MG has a limited variety of DNA-binding proteins (DBP) and transcription factors. To investigate the dynamic changes of the proteomic profile of MG nucleoid, that may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation, a quantitative proteomic study was performed on MG nucleoids obtained from the cell culture in logarithmic and stationary phases of synchronous growth. MG cells were grown in a liquid medium with a 9h starvation period. Nucleoids were obtained from the cell culture at the 26th and the 50th hour (logarithmic and stationary phases respectively) by sucrose density gradient centrifugation. LC-MS analysis was carried out on an Ultimate 3000 RSLCnano HPLC system connected to a Fusion Lumos mass spectrometer, controlled by XCalibur software (ThermoFisher Scientific) via a nanoelectrospray source (Thermo Fisher Scientific). For comprehensive peptide library generation one sample from each biological replicate was run in DDA mode. Then, all the sample were run in a single LC-MS DIA run. Identification of DDA files and DIA quantitation was performed with MaxQuant and Skyline software.
HostingRepository	PRIDE
AnnounceDate	2020-05-12
AnnouncementXML	Submission_2020-07-22_06:59:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandr Zubov
SpeciesList	scientific name: Mycoplasma gallisepticum S6; NCBI TaxID: 1006581;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-10 23:27:40	ID requested
1	2020-05-11 23:15:49	announced
⏵ 2	2020-07-22 06:59:45	announced	2020-07-22: Updated publication reference for PubMed record(s): 32637477.

Zubov Aleksandr I, Semashko Tatiana A, Evsyutina Daria V, Ladygina Valentina G, Kovalchuk Sergey I, Ziganshin Rustam H, Galyamina Maria A, Fisunov Gleb Y, Pobeguts Olga V, in different growth phases. Data Brief, 31():105853(2020) [pubmed]

submitter keyword: mycoplasma gallisepticum, nucleoid, synchronous growth, nucleoid associated proteins, DIA

Gleb Fisunov
contact affiliation	Lab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
contact email	herr.romanoff@gmail.com
lab head
Aleksandr Zubov
contact affiliation	Lab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow
contact email	alexanderzubov@icloud.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD019077

PRIDE project URI

• PRIDE
  1. PXD019077
     1. Label: PRIDE project
     2. Name: Proteomic profile of nucleoid-associated proteins isolated from Mycoplasma gallisepticum in different growth phases