PXD019076

PXD019076 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic profiling of mitochondrial toxicants in human cardiomyocyte cell line
Description	Mitochondria are essential cellular organelles that participate in important cellular processes, including bioenergetics, metabolism, and signaling. Recent functional and proteomic studies have revealed the remarkable complexity of mitochondrial protein organization. Protein machineries with diverse functions such as protein translocation, respiration, metabolite transport, protein quality control and the control of membrane architecture interact with each other in dynamic networks. The goal of this study was to identify protein expression changes in a human cardiomyocyte cell line treated with several mitochondrial toxicants which inhibit mitochondrial membrane potential (MMP) and mitochondrial respiration. AC16 human cardiomyocyte cells were treated with carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), dinoterb, picoxystrobin, pinacyanol and triclocarban for 18 h around the IC50 values generated from MMP assay. The samples were harvested and labeled with tandem mass tags with different mass isotopes. Peptide assignment was performed in Proteome Discoverer. Each dataset was analyzed in Ingenuity Pathway Analysis (IPA). In the proteomic profile, these compounds showed dysregulation of a group of mitochondrial proteins (e.g. NDUA, NDUB, BCS1, CYB5B and SDHF2), as well as proteins involved in lipid metabolism (e.g. CPT, MECR, and LPGAT1), cytoskeleton protein changes (e.g. CROCC, LAMC3, FBLN1 and FMN2) and stress response (e.g. IKBKG, IKBB, SYVN1, SOD2 and CPIN1). Proteomic data from the current study provides key insights into chemical induced cellular pathway dysregulation, supporting the use of proteomic profiling as a sensitive method to further explore molecular functions and disease pathogenesis upon exposure to environmental chemicals.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:43:54.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jon (Jian-Jiang) Hao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-10 23:16:36	ID requested
⏵ 1	2021-09-09 00:43:55	announced

Wei Z, Zhao J, Niebler J, Hao JJ, Merrick BA, Xia M, Quantitative Proteomic Profiling of Mitochondrial Toxicants in a Human Cardiomyocyte Cell Line. Front Genet, 11():719(2020) [pubmed]

submitter keyword: Mitochondrial toxicant, FCCP, dinoterb, picoxystrobin, pinacyanol, triclocarban, global proteomic profiling, pathway enrichment analysis

Menghang Xia
contact affiliation	National Center for Advancing Translational Sciences, National Institutes of Health, MD, USA
contact email	mxia@mail.nih.gov
lab head
Jon (Jian-Jiang) Hao
contact affiliation	Poochon Scientific
contact email	jon.hao@poochonscientific.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019076

PRIDE project URI

• PRIDE
  1. PXD019076
     1. Label: PRIDE project
     2. Name: Quantitative proteomic profiling of mitochondrial toxicants in human cardiomyocyte cell line