PXD019066

PXD019066 is an original dataset announced via ProteomeXchange.

Title	EDEM1 interaction proteomics in HEK293T cells
Description	Misfolded or unfolded proteins from the endoplasmic reticulum (ER) are sent to proteasomal degradation via the Endoplasmic Reticulum Associated Degradation (ERAD) pathway. We provide here a proteomic analysis of the interaction partners for one of the ER mannosidases involved in ERAD, ER degradation-enhancing alpha-mannosidase-like protein 1 (EDEM1) and its mutant, a previously described version of EDEM, lacking its N-terminal disordered region (Δ-EDEM1). Moreover, we also performed a whole proteome analysis for cells overexpressing both, WT and Δ-EDEM1 and investigated the proteins which co-fractionate in a sucrose gradient for both EDEM1 and Δ-EDEM1 and their associated proteins.
HostingRepository	PRIDE
AnnounceDate	2020-06-02
AnnouncementXML	Submission_2020-06-02_01:11:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Munteanu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-10 22:26:46	ID requested
⏵ 1	2020-06-02 01:11:17	announced

Chiritoiu M, Chiritoiu GN, Munteanu CVA, Pastrama F, Ivessa NE, Petrescu SM, EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired. Int J Mol Sci, 21(10):(2020) [pubmed]

submitter keyword: ERAD, EDEM1, ER-phagy, proteasomal degradation, LTQ-Orbitrap Velos Pro

Ștefana M. Petrescu
contact affiliation	Department of Molecular Cell Biology, Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest 17, Romania;
contact email	stefana.petrescu@biochim.ro
lab head
Cristian Munteanu
contact affiliation	Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	cristian.v.a.munteanu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD019066

PRIDE project URI

• PRIDE
  1. PXD019066
     1. Label: PRIDE project
     2. Name: EDEM1 interaction proteomics in HEK293T cells